Jun 1, 1985

Mutascreen, an automated bacterial mutagenicity assay

Mutation Research
K FalckH Vainio

Abstract

Mutascreen is an automated instrument for bacterial mutagenicity testing. The biological principles of the Mutascreen assay are the same as those of the bacterial reverse-mutation assays, like the Ames test, but several operational principles are different. The Mutascreen assay takes place in wells containing only 400 microliter of liquid medium. Also, the dispensing of the liquid medium, the bacterial tester strains, the metabolic activation system (S9), and the test solutions is all performed by a computer-controlled robot according to the user's preprogrammed instructions. The turbidity in up to 200 wells is monitored intermittently over a 24-h period by a vertical-pathway photometer, thereby avoiding measurement problems caused by sedimentation. The data for the resulting growth curves is stored for analysis. The auxotrophic growth pattern is altered characteristically by test solutions that are toxic or contain endogenous growth factor(s), while prototrophic growth is observed earlier in the 24-h period when revertants have been induced by the test solution. To compare the Mutascreen assay with the conventional plate assay, 36 chemicals including known carcinogens and noncarcinogens were tested. Both assays identified the ...Continue Reading

Mentioned in this Paper

Biochemical Pathway
Cell Growth Pattern
Alkalescens-Dispar Group
Mutagenicity Tests
Etiology
Sedimentation Procedure
Growth Factor
Turbidity Measurement
Turbidimetry
Assay

About this Paper

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