Mutation analysis of the cross-reactive epitopes of Japanese encephalitis virus envelope glycoprotein

The Journal of General Virology
Shyan-Song ChiouGwong-Jen Chang

Abstract

Group and serocomplex cross-reactive epitopes have been identified in the envelope (E) protein of several flaviviruses and have proven critical in vaccine and diagnostic antigen development. Here, we performed site-directed mutagenesis across the E gene of a recombinant expression plasmid that encodes the Japanese encephalitis virus (JEV) premembrane (prM) and E proteins and produces JEV virus-like particles (VLPs). Mutations were introduced at I135 and E138 in domain I; W101, G104, G106 and L107 in domain II; and T305, E306, K312, A315, S329, S331, G332 and D389 in domain III. None of the mutant JEV VLPs demonstrated reduced activity to the five JEV type-specific mAbs tested. Substitutions at W101, especially W101G, reduced reactivity dramatically with all of the flavivirus group cross-reactive mAbs. The group and JEV serocomplex cross-reactive mAbs examined recognized five and six different overlapping epitopes, respectively. Among five group cross-reactive epitopes, amino acids located in domains I, II and III were involved in one, five and three epitopes, respectively. Recognition by six JEV serocomplex cross-reactive mAbs was reduced by amino acid substitutions in domains II and III. These results suggest that amino acid r...Continue Reading

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Citations

Dec 24, 2015·Reviews in Medical Virology·Minu NainSudhanshu Vrati
Dec 18, 2013·Microbiology and Immunology·Nozyechi N ChidumayoHiroaki Kariwa
Apr 6, 2013·The Journal of General Virology·Ke-Yu SongCheng-Feng Qin
Aug 15, 2018·Pathogens·Sang-Im Yun, Young-Min Lee
Nov 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ahmed RakibJesus Simal-Gandara
May 4, 2021·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Niti YashvardhiniDeepak Kumar Jha

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