Mutation and a high-throughput screening method for improving the production of Epothilones of Sorangium

Journal of Industrial Microbiology & Biotechnology
Guo-li GongYue-zhong Li

Abstract

The epothilones are highly promising prospective anticancer agents that are produced by the myxobacterium Sorangium cellulosum. We mutated the epothilone producing S. cellulosum strain So0157-2 to improve the production of epothilones. For evaluation in high-throughput of a large number of mutants, we developed a simple microtiter method for primary screening. Using the classical UV-mutation method plus selection pressures, the production capacity was increased about 0.5 approximately 2.5 times the starting strain. The mutants with higher production and different phenotypes were further subjected to recursive protoplast fusions and the fusants products were screened under multi-selection pressure. Furthermore, the production was greatly increased by the genome shuffling. For epothilone B, the production of one fusant was increased about 130 times compared to the starting strain, increasing from 0.8 mg l(-1) to 104 mg l(-1).

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Citations

Jul 17, 2008·Journal of Industrial Microbiology & Biotechnology·Zhi-Jie XiaYue-Zhong Li
Jul 4, 2009·The Journal of Antibiotics·Jidong WangHua Bai
Jul 8, 2010·The Journal of Antibiotics·Chunhua LuYuemao Shen
Sep 9, 2011·Natural Product Research·Ji-Dong WangHua Bai
Mar 7, 2014·Applied Microbiology and Biotechnology·Damien Biot-Pelletier, Vincent J J Martin
May 26, 2009·Biotechnology Advances·Jixian GongXueming Zhao
Feb 16, 2008·Current Opinion in Chemical Biology·Christine Nicole S Santos, Gregory Stephanopoulos
Jun 3, 2009·Journal of Applied Microbiology·Y ChengY Qu
Dec 7, 2013·Microbial Biotechnology·Shu-Guang LiYue-Zhong Li
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Sep 25, 2020·World Journal of Microbiology & Biotechnology·Liang ChenKe Bian
Apr 2, 2018·Biotechnology Letters·Fangxue XuChunchao Han
Nov 10, 2020·Applied Microbiology and Biotechnology·Sandip Ghosh, Bomba Dam

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