Mutation and formation of methyl- and hydroxylguanine adducts in DNA caused by N-nitrosodimethylamine and N-nitrosodiethylamine with UVA irradiation

Carcinogenesis
Sakae Arimoto-KobayashiH Hayatsu

Abstract

Previously we reported that when Escherichia coli was treated with N-nitrosodialkylamine under irradiation with near UV light, mutagenesis of the bacteria took place: there was no requirement for metabolic activation. We have now studied the spectra of mutations caused by N-nitrosodimethylamine (NDMA) and N-nitrosodiethylamine (NDEA) with UVA (320-400 nm) irradiation, using standard tester strains for identifying types of mutations. Induced mutations by NDMA + UVA were the transition GC-->AT and transversions GC-->CG, GC-->TA and AT-->TA. NDEA + UVA induced mainly the GC-->CG transversion. In both cases no frameshift mutations were observed. When O6-alkylguanine-DNA alkyltransferase-deficient strains of E. coli and Salmonella typhimurium were used, the mutation levels with both NDMA + UVA and NDEA + UVA became remarkably higher than those observed with the proficient strains. We measured the O6-methylguanine (O6-meG) level in calf thymus DNA treated with NDMA + UVA. The O6-meG level was increased as a function of NDMA concentration and irradiation time. We also detected N7-methylguanine in DNA treated with NDMA + UVA. In our previous work we found formation of 8-oxodeoxyguanosine (8-oxodG) in DNA treated with N-nitrosomorpholin...Continue Reading

Citations

Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yukihiro EsakaMasashi Goto
Jul 6, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Tomoyasu ToyoizumiNaohide Kinae
Sep 1, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Badar Ul IslamAsif Ali
Mar 29, 2000·Environmental and Molecular Mutagenesis·D H PhillipsK W Turteltaub
Mar 13, 2019·Chemical Communications : Chem Comm·Claudia M N AloisiHailey L Gahlon
Jun 6, 2021·Environmental and Molecular Mutagenesis·George E JohnsonAndreas Zeller
Nov 24, 1999·Journal of Agricultural and Food Chemistry·S Arimoto-KobayashiH Hayatsu

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