PMID: 2500529Apr 20, 1989Paper

Mutation changing the specificity of an RNA polymerase sigma factor

Journal of Molecular Biology
P ZuberR Losick

Abstract

We describe a mutation that changes the fine specificity of promoter selection by a secondary form of RNA polymerase holoenzyme in Bacillus subtilis. The product of regulatory gene spo0H is an RNA polymerase sigma factor called sigma H, which directs transcription of a sporulation gene known as spoVG. We show that the spo0H mutation spo0H81, which blocks transcription from the wild-type spoVG promoter, enhances transcription from a mutant form of the spoVG promoter (spoVG249) bearing a severe down-mutation (a G.C to A.T transition) at position -13 in the "-10 region." Suppression of the spoVG249 mutation is specific in the sense that the transcription from several other spoVG mutant promoters was not restored by the mutant sigma. Evidently, spo0H81 is a change-of-specificity mutation that alters sigma H-RNA polymerase in a way that decreases its capacity to use the wild-type spoVG promoter, while increasing its capacity to use the mutant promoter. Transcription experiments in vitro using RNA polymerase containing the wild-type or mutant sigma support this interpretation. The spo0H81 mutation causes a threonine (Thr100) to isoleucine substitution in a region of sigma H that is highly homologous among sigma factors of diverse ori...Continue Reading

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Citations

Aug 5, 1991·Journal of Molecular Biology·J MecsasC A Gross
Jan 20, 1992·Journal of Molecular Biology·F W Whipple, A L Sonenshein
Jul 31, 1995·Journal of Biotechnology·G Gross, H Hauser
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May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·D M JooR Calendar
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·A HigashitaniK Horiuchi

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