PMID: 8976839Aug 1, 1996Paper

Mutation in DNA gyrase of norfloxacin-resistant clinical isolates of Neisseria gonorrhoeae

Genitourinary Medicine
M TanakaJ Kumazawa

Abstract

Recently a rapid decrease in the susceptibility of Neisseria gonorrhoeae isolates to fluoroquinolones has occurred and gonococcal fluoroquinolone resistance is now a significant problem in the treatment of gonorrhoea in Japan. Thus, in order to investigate the quinolone resistance mechanisms in clinical isolates of N gonorrhoeae we studied an alteration in the DNA gyrase subunit A (GyrA) which is well-known as a common mechanism of bacterial quinolone resistance. Four clinical isolates of N gonorrhoeae resistant to norfloxacin and 5 strains susceptible to norfloxacin, including 2 clinical isolates and 3 WHO reference strains, were tested in this study. To identify mutations in the GyrA genes of gonococcal strains, polymerase chain reaction and direct DNA sequencing were performed. A single base change (serine codon TCC changed to phenylalanine codon TTC), which resulted in an amino acid change in GyrA at position 91, was identified in all 4 norfloxacin-resistant strains for which the MICs of norfloxacin ranged from 1.0 to 8.0 micrograms/ml, while no mutation within GyrA was detected in 5 norfloxacin-susceptible strains for which the MICs of norfloxacin ranged from 0.004 to 0.063 microgram/ml. The results from this study suggest...Continue Reading

References

Feb 1, 1992·Antimicrobial Agents and Chemotherapy·T E ClendennenF S Wignall
Dec 1, 1991·The Journal of Antimicrobial Chemotherapy·H IshiiY Osada
Jan 20, 1990·Lancet·A E Jephcott, A Turner
Jan 1, 1983·Methods in Enzymology·J Messing
Feb 1, 1995·Antimicrobial Agents and Chemotherapy·T DeguchiY Kawada
Apr 1, 1993·Antimicrobial Agents and Chemotherapy·H YoshidaS Nakamura
Oct 1, 1995·Antimicrobial Agents and Chemotherapy·M TanakaJ Kumazawa

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Citations

Jun 20, 1998·Sexually Transmitted Infections·M TanakaJ Kumazawa
Apr 29, 2006·International Journal of Urology : Official Journal of the Japanese Urological Association·Katsumi ShigemuraMasato Fujisawa
Apr 10, 2019·BMC Microbiology·Mary Wandia KivataJames D Mancuso
Sep 11, 2020·Biomedical Chromatography : BMC·Nathália R SantosJosé A Menezes-Filho

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