Mutation in the LPS outer core biosynthesis gene, galU, affects LPS interaction with the RTX toxins ApxI and ApxII and cytolytic activity of Actinobacillus pleuropneumoniae serotype 1

Molecular Microbiology
Mahendrasingh RamjeetMario Jacques

Abstract

Lipopolysaccharides (LPS) and Apx toxins are major virulence factors of Actinobacillus pleuropneumoniae, a pathogen of the respiratory tract of pigs. Here, we evaluated the effect of LPS core truncation in haemolytic and cytotoxic activities of this microorganism. We previously generated a highly attenuated galU mutant of A. pleuropneumoniae serotype 1 that has an LPS molecule lacking the GalNAc-Gal II-Gal I outer core residues. Our results demonstrate that this mutant exhibits wild-type haemolytic activity but is significantly less cytotoxic to porcine alveolar macrophages. However, no differences were found in gene expression and secretion of the haemolytic and cytotoxic toxins ApxI and ApxII, both secreted by A. pleuropneumoniae serotype 1. This suggests that the outer core truncation mediated by the galU mutation affects the toxins in their cytotoxic activities. Using both ELISA and surface plasmon resonance binding assays, we demonstrate a novel interaction between LPS and the ApxI and ApxII toxins via the core oligosaccharide. Our results indicate that the GalNAc-Gal II-Gal I trisaccharide of the outer core is fundamental to mediating LPS/Apx interactions. The present study suggests that a lack of binding between LPS and ...Continue Reading

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Citations

Feb 11, 2015·BioMed Research International·Rosa Estela Quiroz-CastañedaEdgar Dantán-González
May 3, 2011·Veterinary Microbiology·Alexandra GrasteauMario Jacques
Aug 5, 2009·Molecular Microbiology·Onkar SharmaWilliam A Cramer
Dec 18, 2009·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Aug 27, 2013·Veterinary Microbiology·Bin ZhangHua Yue
Jan 25, 2017·Journal of Enzyme Inhibition and Medicinal Chemistry·Agustín ZavalaMarta Mollerach
Sep 12, 2017·Toxins·Nicole Guiso
Nov 8, 2017·Animal Health Research Reviews·Skander HathroubiMario Jacques
Sep 10, 2009·Veterinary Research·Josée LabrieMario Jacques

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