Mutation in Torenia fournieri Lind. UFO homolog confers loss of TfLFY interaction and results in a petal to sepal transformation

The Plant Journal : for Cell and Molecular Biology
Katsutomo SasakiNorihiro Ohtsubo

Abstract

We identified a Torenia fournieri Lind. mutant (no. 252) that exhibited a sepaloid phenotype in which the second whorls were changed to sepal-like organs. This mutant had no stamens, and the floral organs consisted of sepals and carpels. Although the expression of a torenia class B MADS-box gene, GLOBOSA (TfGLO), was abolished in the 252 mutant, no mutation of TfGLO was found. Among torenia homologs such as APETALA1 (AP1), LEAFY (LFY), and UNUSUAL FLORAL ORGANS (UFO), which regulate expression of class B genes in Arabidopsis, only accumulation of the TfUFO transcript was diminished in the 252 mutant. Furthermore, a missense mutation was found in the coding region of the mutant TfUFO. Intact TfUFO complemented the mutant phenotype whereas mutated TfUFO did not; in addition, the transgenic phenotype of TfUFO-knockdown torenias coincided with the mutant phenotype. Yeast two-hybrid analysis revealed that the mutated TfUFO lost its ability to interact with TfLFY protein. In situ hybridization analysis indicated that the transcripts of TfUFO and TfLFY were partially accumulated in the same region. These results clearly demonstrate that the defect in TfUFO caused the sepaloid phenotype in the 252 mutant due to the loss of interaction ...Continue Reading

References

Aug 1, 1990·The Plant Cell·V F Irish, I M Sussex
Jan 1, 1989·The Plant Cell·J L BowmanE M Meyerowitz
Jul 1, 1994·Genes & Development·K Goto, E M Meyerowitz
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·J L RiechmannE M Meyerowitz
Feb 1, 1997·Current Biology : CB·I LeeD Weigel
Oct 23, 1998·Nature·F ParcyD Weigel
Oct 30, 1998·Genes & Genetic Systems·N ShikazonoS Tano
Dec 28, 1999·Annual Review of Cell and Developmental Biology·R J Deshaies
Apr 3, 2001·The Plant Cell·M Ng, M F Yanofsky
Aug 1, 1991·The Plant Cell·E. A. Schultz, G. W. Haughn
Jun 27, 2003·Proceedings of the National Academy of Sciences of the United States of America·Tim DurfeePatricia C Zambryski
Nov 15, 2003·Molecular Phylogenetics and Evolution·Annette Becker, Günter Theissen
Jan 12, 2005·Journal of Experimental Botany·Naoya ShikazonoAtsushi Tanaka
Jul 31, 2007·Trends in Plant Science·Douglas E SoltisJim Leebens-Mack
Aug 2, 2007·The Plant Journal : for Cell and Molecular Biology·Kyoko IkedaYasuo Nagato
Sep 24, 1993·Science·D Weigel, E M Meyerowitz
Nov 21, 2008·PLoS Biology·Zachary B LippmanDani Zamir
Sep 28, 2010·Molecular Genetics and Genomics : MGG·Katsutomo SasakiNorihiro Ohtsubo

❮ Previous
Next ❯

Citations

Apr 4, 2014·BMC Plant Biology·Masahiro NishiharaTakashi Nakatsuka
Jan 22, 2013·Journal of Integrative Plant Biology·Zhenhua WangJun Yang
Mar 2, 2019·International Journal of Molecular Sciences·Naoko FujitaKen Komatsu
Sep 13, 2013·Genes & Genetic Systems·Yusuke KazamaTomoko Abe
Mar 20, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yue ChenGang Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.