PMID: 7010138Feb 1, 1981Paper

Mutation induction in Haemophilus influenzae by ICR-191. I. Development of a detection system for frameshift mutations

Mutation Research
S W PerdueP C McGray

Abstract

The investigation of mutagenic mechanisms in Haemophilus influenzae has been confined until now to mutagens that normally produce mainly base pair substitutions. This paper describes the development of a system suitable for detecting frameshift mutations induced by ICR-191. The system involves reversions from thymidine dependence to thymidine independence. Evidence is presented from a comparison of the responses to ICR-191 and to N-methyl-N'-nitro-N-nitrosoguanidine that the system is specific for frameshift mutations. The genetic recombination involved in transformation leads to a marked increase in "spontaneous" reversion of the frameshift mutations but not of the base substitution mutations. Presumably, this is a consequence of mispairing, with consequent change in the number of bases, during the recombination.

References

Jul 21, 1972·Journal of Molecular Biology·J K SetlowR F Kimball
Jan 1, 1974·Annual Review of Genetics·J R Roth

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