Mutation of G51 in SepF impairs FtsZ assembly promoting ability of SepF and retards the division of Mycobacterium smegmatis cells

The Biochemical Journal
Dipanwita BhattacharyaDulal Panda

Abstract

The role of FtsZ-associated proteins in the regulation of the assembly dynamics of Mycobacterium smegmatis FtsZ is not clear. In this work, we examined the effect of M. smegmatis SepF on the assembly and stability of M. smegmatis FtsZ polymers. We discovered a single dominant point mutation in SepF (G51D or G51R) that renders the protein inactive. SepF promoted the polymerization of FtsZ, induced the bundling of FtsZ filaments, stabilized FtsZ filaments and reduced the GTPase activity of FtsZ. Surprisingly, both G51D-SepF and G51R-SepF neither stabilized FtsZ filaments nor showed a discernable effect on the GTPase activity of FtsZ. The binding affinity of SepF to FtsZ was found to be stronger than the binding affinity of G51R/D-SepF to FtsZ. Interestingly, the binding affinity of SepF to G51R-SepF was determined to be 45 times stronger than FtsZ. In addition, the interaction of SepF with G51R-SepF was found to be 2.6 times stronger than SepF-SepF interaction. Furthermore, G51R-SepF impaired the ability of SepF to promote the assembly of FtsZ. In addition, the overexpression of G51R-SepF in M. smegmatis mc2 155 cells retarded the proliferation of these cells and increased the average length of the cells. The results indicated th...Continue Reading

References

Jan 1, 1991·Analytical Biochemistry·T P GeladopoulosA E Evangelopoulos
Feb 10, 1995·Cell·H P Erickson
Feb 28, 1998·The EMBO Journal·A Mukherjee, J Lutkenhaus
Feb 16, 2002·The EMBO Journal·Sebastien Pichoff, Joe Lutkenhaus
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jesse StrickerHarold P Erickson
Mar 26, 2003·Annual Review of Biochemistry·Kathleen R Ryan, Lucy Shapiro
Apr 23, 2004·Molecular Microbiology·Daniel P HaeusserPetra Anne Levin
Mar 9, 2005·Molecular Microbiology·Sebastien Pichoff, Joe Lutkenhaus
Jan 20, 2006·Molecular Microbiology·Leendert W HamoenJeff Errington
Apr 9, 2008·Molecular Microbiology·Gitte EbersbachKenn Gerdes
Apr 26, 2008·Protein Science : a Publication of the Protein Society·Richa Jaiswal, Dulal Panda
Aug 15, 2009·Nature Reviews. Microbiology·David W Adams, Jeff Errington
Sep 26, 2009·The EMBO Journal·Masaki OsawaHarold P Erickson
Jan 11, 2011·Journal of Bacteriology·Jorge M Durand-HerediaAnuradha Janakiraman
Jan 13, 2011·The EMBO Journal·Muhammet E GündoğduLeendert W Hamoen
Mar 22, 2012·Biochemistry·Yaodong ChenHarold P Erickson
Apr 17, 2012·Journal of Bacteriology·Jorge Durand-HerediaAnuradha Janakiraman
Aug 23, 2012·PloS One·Ewa CendrowiczDirk-Jan Scheffers
Feb 8, 2013·Molecular Microbiology·Bhupender SinghSantanu Dasgupta
Nov 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ramona DumanLeendert W Hamoen
May 7, 2015·Molecular Microbiology·Susanne GolaMiguel Vicente
Aug 19, 2015·International Journal of Biological Macromolecules·Anusri BhattacharyaDulal Panda
Sep 10, 2016·Scientific Reports·Daniel O DaleyBill Söderström
Nov 23, 2016·International Journal of Biological Macromolecules·Dipanwita BhattacharyaDulal Panda
Feb 18, 2017·Science·Alexandre W Bisson-FilhoEthan C Garner
Jun 22, 2017·Endocrine-related Cancer·Rachana Rao Battaje, Dulal Panda
Dec 7, 2017·Scientific Reports·Yongqiang GaoLeendert W Hamoen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved