Mutation of Hip's carboxy-terminal region inhibits a transitional stage of progesterone receptor assembly.

Molecular and Cellular Biology
V PrapapanichDavid F Smith

Abstract

Steroid receptor complexes are assembled through an ordered, multistep pathway involving multiple components of the cytoplasmic chaperone machinery. Two of these components are Hsp70-binding proteins, Hip and Hop, that have some limited homology in their C-terminal regions, outside the sequences mapped for Hsp70 binding. Within this region of Hip is a DPEV sequence that occurs twice; in Hop, one DPEV sequence plus a partial second sequence occurs. In an effort to better understand Hip function as it relates to assembly of progesterone receptor complexes, the DPEV region of Hip was targeted for mutations. Each DPEV sequence was mutated to an APAV sequence, singly or in combination. The combined mutation, APAV2, was further combined with a deletion of Hip's tetratricopeptide repeat region that is required for Hsp70 binding or with a deletion of Hip's GGMP repeat. An additional mutant was prepared by truncation of Hip's DPEV-containing C terminus. By comparing interactions of various Hip forms with Hsp70, it was determined that mutation of the DPEV sequences created a dominant inhibitory form of Hip. The mutant Hip-Hsp70 complex was not prevented from interacting with progesterone receptor, but the mutant caused a dose-dependent i...Continue Reading

References

Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·L WhitesellL M Neckers
Mar 1, 1994·Molecular and Cellular Biology·J L JohnsonD O Toft
Dec 1, 1995·Molecular and Cellular Biology·D F SmithR A Rimerman
May 31, 1996·The Journal of Biological Chemistry·K D DittmarW B Pratt
Jul 5, 1996·The Journal of Biological Chemistry·M C O'BrienD B McKay
Nov 1, 1996·Molecular and Cellular Biology·V PrapapanichD F Smith
Jan 1, 1997·Molecular and Cellular Biology·H C ChangS Lindquist
Jan 24, 1997·The Journal of Biological Chemistry·H Irmer, J Höhfeld
Mar 1, 1997·Trends in Biochemical Sciences·J Frydman, J Höhfeld

❮ Previous
Next ❯

Citations

Dec 18, 1998·The Journal of Biological Chemistry·S Chen, D F Smith
Aug 6, 2002·The Journal of Biological Chemistry·M Patricia HernándezDavid O Toft
Feb 4, 2003·Experimental Biology and Medicine·William B Pratt, David O Toft
Feb 27, 2007·Journal of Zhejiang University. Science. B·Zheng-zheng ShiShu Zheng
Nov 6, 2012·Journal of Zhejiang University. Science. B·Rui BaiShu Zheng
Nov 15, 2000·The Journal of Biological Chemistry·E A NollenH H Kampinga
Oct 23, 2004·Cell Stress & Chaperones·David F Smith
Jun 8, 2001·Annual Review of Biochemistry·J Frydman
Oct 16, 2001·Cell Stress & Chaperones·M A WebbG A Thompson
Nov 1, 2001·The Journal of Biological Chemistry·Marion VeltenMoncef M Ladjimi
Jan 26, 2002·The Journal of Biological Chemistry·M Patricia HernándezDavid O Toft
Apr 9, 2004·Molecular Endocrinology·Gregory M NelsonDavid F Smith
Dec 26, 2001·The Journal of Biological Chemistry·Guo-Huang FanAnn Richmond
Dec 15, 2010·Cell Stress & Chaperones·Rudi Kenneth Allan, Thomas Ratajczak
Jan 16, 1999·The Journal of Biological Chemistry·F VázquezM L Iruela-Arispe
Jan 5, 1999·The Journal of Biological Chemistry·S TakayamaJ C Reed
Jul 21, 2006·The Journal of Biological Chemistry·Nela S Cintron, David Toft
Dec 8, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Rongmin Zhao, Walid A Houry
Nov 25, 2003·Cell Stress & Chaperones·Gregory M NelsonDavid F Smith
Sep 27, 2002·The Journal of Biological Chemistry·William P SullivanDavid O Toft
Jan 6, 2005·The Journal of Biological Chemistry·Patricia E CarriganDavid F Smith
Feb 24, 2021·Protein Science : a Publication of the Protein Society·Michelle Y FryWilliam M Clemons
Mar 29, 2000·Seminars in Cell & Developmental Biology·D F Smith
Dec 2, 2004·Biochimica Et Biophysica Acta·Claudia EsserJörg Höhfeld
May 27, 2009·Journal of Molecular Biology·Hyun-Ok SongJoohong Ahnn
Apr 21, 2010·Molecular and Biochemical Parasitology·Pablo C EcheverriaSergio O Angel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.