Mutation of mouse p53 Ser23 and the response to DNA damage.

Molecular and Cellular Biology
Zhiqun WuYang Xu

Abstract

Recent studies have suggested that phosphorylation of human p53 at Ser20 is important for stabilizing p53 in response to DNA damage through disruption of the interaction between MDM2 and p53. To examine the requirement for this DNA damage-induced phosphorylation event in a more physiological setting, we introduced a missense mutation into the endogenous p53 gene of mouse embryonic stem (ES) cells that changes serine 23 (S23), the murine equivalent of human serine 20, to alanine (A). Murine embryonic fibroblasts harboring the p53(S23A) mutation accumulate p53 as well as p21 and Mdm2 proteins to normal levels after DNA damage. Furthermore, ES cells and thymocytes harboring the p53(S23A) mutation also accumulate p53 protein to wild-type levels and undergo p53-dependent apoptosis similarly to wild-type cells after DNA damage. Therefore, phosphorylation of murine p53 at Ser23 is not required for p53 responses to DNA damage induced by UV and ionizing radiation treatment.

References

Jul 5, 1991·Science·M HollsteinC C Harris
Jan 1, 1994·Current Biology : CB·T JacksR A Weinberg
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S J UllrichE Appella
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J ChenF W Alt
May 1, 1996·Genes & Development·L J Ko, C Prives
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·S K BronsonO Smithies
May 15, 1997·Nature·Y HauptM Oren
May 15, 1997·Nature·M H KubbutatK H Vousden
Jul 1, 1998·Advances in Immunology·J R Gorman, F W Alt
Sep 23, 1998·Genes & Development·K SakaguchiE Appella
Feb 18, 1999·Molecular and Cellular Biology·M AshcroftK H Vousden
May 26, 1999·The Journal of Pathology·C Prives, P A Hall
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·N H ChehabT D Halazonetis
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·M Uesugi, G L Verdine
Sep 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·S MatsuokaS J Elledge
Oct 12, 2000·Nature Cell Biology·R MelchionnaC H McGowan
Oct 18, 2000·Proceedings of the National Academy of Sciences of the United States of America·C ChaoY Xu
May 19, 2001·European Journal of Biochemistry·E Appella, C W Anderson

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Citations

Dec 4, 2002·Journal of Cellular Biochemistry·Susan HauptYgal Haupt
Jan 12, 2005·The Journal of Pathology·Philip J CoatesEric G Wright
Dec 7, 2002·Trends in Biochemical Sciences·Carina I HolmbergLea Sistonen
Dec 19, 2007·Cell Research·Helena Lobo BorgesJean Y J Wang
Apr 21, 2010·Cell Research·Christopher L Brooks, Wei Gu
Nov 28, 2006·Nature Reviews. Cancer·Franck Toledo, Geoffrey M Wahl
Sep 5, 2009·Nature Reviews. Cancer·David W Meek
Apr 3, 2007·Oncogene·T Iwakuma, G Lozano
Dec 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Mila JankovicMichel C Nussenzweig
Oct 3, 2002·The EMBO Journal·Hiroyuki TakaiNoboru Motoyama
Jul 23, 2008·Molecular and Cellular Biology·Christine M LovlyHelen Piwnica-Worms
Jul 9, 2002·Molecular and Cellular Biology·Harry A RogoffTimothy F Kowalik
Oct 1, 2004·Molecular and Cellular Biology·Wendy BruinsAnnemieke de Vries
Nov 16, 2004·Molecular and Cellular Biology·Francisco M VegaPedro A Lazo
Jan 20, 2004·Molecular and Cellular Biology·Hayla K SlussStephen N Jones
Mar 23, 2004·Annual Review of Immunology·Yun-Cai Liu
Apr 30, 2008·Neuro-oncology·Akira NakamizoFrederick F Lang
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mark A McCoyDaniel F Wyss
Feb 23, 2010·Trends in Cell Biology·Mark WadeGeoffrey M Wahl
May 12, 2015·The Journal of Reproduction and Development·Takashi TanakaTakashi Shinohara
Nov 7, 2015·Cell Stem Cell·Jun Wu, Juan Carlos Izpisua Belmonte
Sep 3, 2004·The EMBO Journal·David MacPhersonTyler Jacks
Feb 8, 2011·The EMBO Journal·Takao KurodaJunn Yanagisawa
Jan 8, 2014·Seminars in Cell & Developmental Biology·Patty B Garcia, Laura D Attardi
Nov 26, 2009·Critical Reviews in Biochemistry and Molecular Biology·Karen A Boehme, Christine Blattner
Jan 31, 2003·Expert Opinion on Therapeutic Targets·Hayla K Sluss, Stephen N Jones
May 20, 2009·Cell·Jan-Philipp Kruse, Wei Gu
Jun 8, 2013·Biochimica Et Biophysica Acta·Andreas K Hock, Karen H Vousden
May 20, 2008·Cell·Yi TangWei Gu
Oct 25, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zheng TaoHaitao Ma
Mar 3, 2004·Nature Reviews. Cancer·Bin-Bing S Zhou, Jiri Bartek
Aug 12, 2003·The Journal of Biological Chemistry·Connie ChaoYang Xu

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