Abstract
Rapid proliferation of squamous cell carcinomas of the head and neck (SCCHN) during therapy may contribute to treatment failure. We have investigated the presence of p53 abnormalities in patients with SCCHN as a correlate of proliferation rate and other pathologic and clinical variables. p53 Mutation, as determined by polymerase chain reaction and single-strand conformation polymorphism analysis of microdissected frozen sections of tumor biopsies, was significantly associated with a high labeling index, as determined by in vivo infusion of IUdR and BrdU (P = 0.017). p53 Protein expression was detected by immunohistochemistry with two different antibodies, followed by quantitative image analysis. Many cases exhibited strong p53 protein expression in the absence of mutations within the conserved region of the gene, and expression was not related to proliferation. The presence of p53 mutations was related to tumor differentiation in this group of patients.
References
May 3, 1991·Science·D SidranskyP Frost
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G GaidanoR Dalla-Favera
Dec 1, 1990·International Journal of Radiation Oncology, Biology, Physics·A C BeggM Van Glabbeke
Jun 1, 1994·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·L XuP Garin-Chesa
Oct 1, 1993·Archives of Otolaryngology--head & Neck Surgery·N K LeeP D Nisen
Sep 24, 1993·Cell·S W LoweD E Housman
Aug 15, 1987·Applied Optics·J W Bacus, L J Grace
Citations
Jun 16, 2001·Head & Neck·M FriedmanJ S Coon
Jul 20, 2005·Journal of Cancer Research and Clinical Oncology·D DworakowskaE Czestochowska
Nov 7, 2001·The American Journal of Pathology·S L HolleyP R Hoban
Mar 15, 2002·Auris, Nasus, Larynx·Christiane KuropkatJ A Werner
Jun 7, 2000·Current Opinion in Oncology·A N Salesiotis, K J Cullen
May 20, 1998·The Laryngoscope·L C WhislerJ S Coon
Jan 22, 2002·The Laryngoscope·P LavertuM Secic
Oct 29, 2002·Journal of Clinical Pathology·I CruzC J L M Meijer
Oct 21, 2000·Molecular Pathology : MP·H K Williams
Apr 24, 2012·ISRN Surgery·Eric J Yavrouian, Uttam K Sinha
Jan 16, 2004·Head & Neck·Juan Carlos de VicenteJuan Sebastián López Arranz
Feb 18, 2009·Journal of Cutaneous Pathology·Mulazim H BukhariNaseer A Chaudhry
Apr 4, 2002·Pathology, Research and Practice·L RubioF J Vera-Sempere
Feb 15, 2011·American Journal of Otolaryngology·YuLi XiaoDeJun Jin
Oct 19, 2002·Oncogene·Yang GuoMasahiro Hitomi
Aug 30, 2003·Oncogene·Bruce G Haffty, Peter M Glazer
Nov 9, 2017·International Journal of Oncology·Yasutaka YamadaNaohiko Seki