Mutation of the GKS motif of the RNA-dependent RNA polymerase from potato virus X disables or eliminates virus replication

The Journal of General Virology
G F Davenport, D C Baulcombe

Abstract

The RNA-dependent RNA polymerase (RdRp) of potato virus X (PVX) contains a glycine-lysine-serine (GKS) motif. This motif is present in the replication enzyme of many RNA viruses and is thought to be required for nucleoside triphosphate-binding. Three single amino acid changes, glycine to alanine (AKS), lysine to asparagine (GNS) and lysine to glutamate (GES) within the GKS motif of the PVX RdRp were tested for their effect on PVX accumulation. The GNS and GES mutations rendered the virus unable to accumulate in either tobacco plants or protoplasts, whereas substitution of glycine with alanine had only a minor effect on accumulation of PVX. The glycine to alanine mutation reverted to wild-type after passage on Nicotiana clevelandii plants. These findings suggest that the GKS motif is required for PVX replication and that strong selection pressures are active to maintain necessary sequences of the viral RdRp.

Citations

Dec 5, 2015·Frontiers in Plant Science·Chiara LicoSelene Baschieri
Nov 1, 2005·Virus Research·Marta Gómez de CedrónJuan Antonio García
Sep 5, 2009·Virology·Devinka BamunusingheJeanmarie Verchot-Lubicz
May 18, 2000·Virology·D J Lewandowski, W O Dawson
May 3, 2003·Virology·Konduru KrishnamurthyJeanmarie Verchot-Lubicz
Mar 17, 2007·Annual Review of Phytopathology·Giovanni P MartelliValerian V Dolja
Dec 18, 2010·BMC Biotechnology·Zun Liu, Christopher M Kearney
Aug 13, 2005·Archives of Virology·I E TzanetakisR R Martin
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Dec 7, 2007·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Fabien CôtéDenis Leclerc

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