Mutation of waaC, encoding heptosyltransferase I in Campylobacter jejuni 81-176, affects the structure of both lipooligosaccharide and capsular carbohydrate.

Journal of Bacteriology
Margaret I KanipesMario A Monteiro

Abstract

Campylobacter jejuni 81-176 lipooligosaccharide (LOS) is composed of two covalently linked domains: lipid A, a hydrophobic anchor, and a nonrepeating core oligosaccharide, consisting of an inner and outer core region. We report the isolation and characterization of the deepest rough C. jejuni 81-176 mutant by insertional mutagenesis into the waaC gene, encoding heptosyltransferase I that catalyzes the transfer of the first L-glycero-D-manno-heptose residue to 3-deoxy-D-manno-octulosonic residue (Kdo)-lipid A. Tricine gel electrophoresis, followed by silver staining, showed that site-specific mutation in the waaC gene resulted in the expression of a severely truncated LOS compared to wild-type strain 81-176. Gas-liquid chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy showed that the waaC LOS species lacked all sugars distal to Kdo-lipid A. Parallel structural studies of the capsular polysaccharides of the wild-type strain 81-176 and waaC mutant revealed loss of the 3-O-methyl group in the waaC mutant. Complementation of the C. jejuni mutant by insertion of the wild-type C. jejuni waaC gene into a chromosomal locus resulted in LOS and capsular structures identical to those expressed in the parent strai...Continue Reading

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Citations

Jul 13, 2012·The Journal of Biological Chemistry·Matthew McCallumCarole Creuzenet
Dec 31, 2008·Infection and Immunity·Mario A MonteiroPatricia Guerry
Dec 25, 2007·Journal of Bacteriology·Margaret I KanipesMario A Monteiro
Aug 19, 2008·Trends in Microbiology·Patricia Guerry, Christine M Szymanski
Sep 4, 2014·Microbiology and Molecular Biology Reviews : MMBR·Hanne L P Tytgat, Sarah Lebeer
Sep 1, 2007·Current Opinion in Infectious Diseases·Nick Dorrell, Brendan W Wren
Aug 7, 2007·The Journal of Biological Chemistry·David J McNallyChristine M Szymanski
Jun 7, 2014·Journal of Animal Science·C W ForsbergJ P Phillips
Mar 12, 2021·Natural Product Reports·Zhengyan GuoYihua Chen
Jun 25, 2020·ACS Infectious Diseases·Maude Cloutier, Charles Gauthier
Aug 12, 2008·Carbohydrate Research·Jianqiang Zhao, Mario A Monteiro

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