Mutation pattern in rifampicin resistance determining region of rpoB gene in multidrug-resistant Mycobacterium tuberculosis isolates from Pakistan

International Journal of Mycobacteriology
Obaidullah QaziSehrish Firyal

Abstract

The current study was undertaken to characterize the RRDR rpoB gene mutations among the rifampicin-resistant Mycobacterium tuberculosis (MTB) isolates from Pakistan. Rifampicin mutation patterns were analyzed by using PCR followed by rpoB gene sequencing. Among the 1080 referred TB cases, 63 (6%) were resistant against at least one first-line TB drug. Out of these 63 resistant isolates, 24 isolates (38%) were found to be resistant to isoniazid and rifampicin. Sequence analysis of multidrug-resistant tuberculosis (MDR-TB) isolates detected a single mutation in the RRDR region of the rpoB gene at codon 531, 516, 512, 528 and 533; however, 5 MDR-TB isolates lack any mutation in the RRDR region. A double mutation was observed in 1 MDR-TB isolate at codon 512 and 516 which are reported for the first time from Pakistan. Moreover, in 1 isolate a novel silent mutation was observed at codon 528. Further studies about these mutations may be helpful in the development of diagnostic tools for the detection of MTB in a high TB endemic area like Pakistan.

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Citations

Dec 15, 2015·International Journal of Mycobacteriology·Fatima A KhalidM M Mukhtar
Feb 28, 2018·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Azar Dokht KhosraviMehdi Mirsaeidi
Jul 11, 2021·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Mehmood QadirRani Faryal
Jun 6, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zubair Shanib BhatZahoor Ahmad

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