Mutational analyses of regulatory genes, mexR, nalC, nalD and mexZ of mexAB-oprM and mexXY operons, in efflux pump hyperexpressing multidrug-resistant clinical isolates of Pseudomonas aeruginosa

World Journal of Microbiology & Biotechnology
Manju SureshP R Manish Kumar

Abstract

The present study deals with membrane-bound efflux pumps, MexAB-OprM and MexXY and their respective regulatory genes mexR, nalC, nalD and mexZ in multidrug resistant (MDR) Pseudomonas aeruginosa. Following antibiotic sensitivity testing and detection of various beta-lactamases, hyperexpression of efflux pump genes, mexB and mexY in the isolates was investigated using semi-quantitative and real-time reverse transcription-PCR. Amplicons from regulatory genes were sequenced and subjected to mutational and phylogenetic analysis. Twenty-nine clinical isolates of P. aeruginosa were obtained from a total of 144 MDR gram-negative bacteria collected from Kerala State, South India. All strains were found to be resistant to ampicillin and nalidixic acid with 13.8, 44.8 and 31% testing positive for extended-spectrum beta-lactamases, metallo-beta-lactamases and AmpC producers respectively. Increased mexB and mexY transcription was detected respectively in 10.3 and 20.7% of the isolates in comparison with P. aeruginosa reference strain, PAO (MTCC). Co-expression of MexY was also observed in MexB overproducers. Various synonymous/and non-synonymous mutations in regulatory gene sequences of efflux pump operons were detected. In the strain desi...Continue Reading

References

Aug 29, 2001·Bioinformatics·J P Huelsenbeck, F Ronquist
May 30, 2002·The Journal of Biological Chemistry·Daniel LimNatalie C J Strynadka
Jan 7, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·K Poole
Apr 24, 2004·Antimicrobial Agents and Chemotherapy·Catherine LlanesPatrick Plésiat
Sep 18, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S IslamB Wretlind
Dec 14, 2004·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Sudhindra R GadagkarSudhir Kumar
Jul 18, 2006·Nature Reviews. Microbiology·Laura J V Piddock
Apr 10, 2008·Molecular Biology and Evolution·David Posada
Sep 2, 2008·Applied and Environmental Microbiology·Nurul Huda KhanKazuhiro Kogure
Sep 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Hao ChenChuan He
Sep 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Mark S WilkeNatalie C J Strynadka
Jun 17, 2009·Journal of Medical Microbiology·Tanya Strateva, Daniel Yordanov
Dec 18, 2009·Expert Review of Anti-infective Therapy·Alexandre P ZavasckiAna C Gales
Aug 10, 2010·Journal of Structural Biology·Yilmaz AlguelXiaodong Zhang
May 20, 2011·The Libyan Journal of Medicine·Momen AskouraIbrahim Taher
Jul 13, 2011·Frontiers in Microbiology·Keith Poole
Nov 28, 2012·Indian Journal of Medical Microbiology·R ChoudhuryD V Singh
Dec 13, 2012·Frontiers in Microbiology·Yuji MoritaYoshiaki Kawamura
Apr 25, 2013·Chemical Biology & Drug Design·Samad Jahandideh
Jun 10, 2014·Canadian Journal of Microbiology·Kanchana PoonsukRungtip Chuanchuen
Mar 20, 2015·Clinical Microbiology Reviews·Xian-Zhi LiHiroshi Nikaido
Dec 22, 2015·International Journal of Medical Microbiology : IJMM·Maitrayee ChatterjeeRaja Biswas
Jan 30, 2016·Antimicrobial Agents and Chemotherapy·L F MatasejeJ D Pitout
Apr 10, 2016·Archives of Microbiology·Ya-Ping PanJi-Lu Shen

❮ Previous
Next ❯

Citations

Feb 23, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Manju SureshManish Kumar Panickassery Ramakrishnan

❮ Previous
Next ❯

Datasets Mentioned

BETA
AE004091.2
CP004061.1
CP008739.2
CP012901.1
CP021380.1
and
CP007147.1

Methods Mentioned

BETA
reverse transcription-PCR
PCR

Software Mentioned

MrBayes
MEGA6
MedCalc
BLASTN
clustalW
jModelTest

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.