Mutational Analyses of the Enzymes Involved in the Metabolism of Hydrogen by the Hyperthermophilic Archaeon Pyrococcus furiosus.

Frontiers in Microbiology
Gerrit J SchutMichael W W Adams

Abstract

Pyrococcus furiosus grows optimally near 100°C by fermenting carbohydrates to produce hydrogen (H(2)) or, if elemental sulfur (S(0)) is present, hydrogen sulfide instead. It contains two cytoplasmic hydrogenases, SHI and SHII, that use NADP(H) as an electron carrier and a membrane-bound hydrogenase (MBH) that utilizes the redox protein ferredoxin. We previously constructed deletion strains lacking SHI and/or SHII and showed that they exhibited no obvious phenotype. This study has now been extended to include biochemical analyses and growth studies using the ΔSHI and ΔSHII deletion strains together with strains lacking a functional MBH (ΔmbhL). Hydrogenase activity in cytoplasmic extracts of various strains demonstrate that SHI is responsible for most of the cytoplasmic hydrogenase activity. The ΔmbhL strain showed no growth in the absence of S(0), confirming the hypothesis that, in the absence of S(0), MBH is the only enzyme that can dispose of reductant (in the form of H(2)) generated during sugar oxidation. Under conditions of limiting sulfur, a small but significant amount of H(2) was produced by the ΔmbhL strain, showing that SHI can produce H(2) from NADPH in vivo, although this does not enable growth of ΔmbhL in the absen...Continue Reading

Citations

Apr 29, 2015·International Journal of Molecular Sciences·Yoon-Jung MoonYoung-Ho Chung
Dec 3, 2014·Biochimica Et Biophysica Acta·John W PetersMichael W W Adams
Aug 19, 2015·Frontiers in Microbiology·Sebastiaan K SpaansServé W M Kengen
Jun 3, 2016·Scientific Reports·Grégoire Michoud, Mohamed Jebbar
Mar 16, 2017·Molecular Microbiology·Gina L LipscombMichael W W Adams
Jun 27, 2018·FEMS Microbiology Reviews·Christopher T StraubRobert M Kelly
Apr 15, 2014·Applied and Environmental Microbiology·Christian BeimgrabenJens Appel
Mar 7, 2014·Microbiology and Molecular Biology Reviews : MMBR·Christopher BräsenBettina Siebers
Jan 20, 2019·Applied and Environmental Microbiology·Sarah Piché-Choquette, Philippe Constant
May 5, 2017·World Journal of Microbiology & Biotechnology·Preeti Ranawat, Seema Rawat
May 18, 2020·Journal of Bioscience and Bioengineering·Jan-Robert SimonsHaruyuki Atomi
Sep 7, 2021·Frontiers in Microbiology·Dominik K Haja, Michael W W Adams

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