Mutational analyses of the human Rad51-Tyr315 residue, a site for phosphorylation in leukaemia cells

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
Yoshimasa TakizawaHitoshi Kurumizaka

Abstract

The human Rad51 protein, which plays a central role in homologous recombination, catalyses homologous pairing. The Rad51-Tyr315 residue is known to be constitutively phosphorylated in leukaemia cells and is thought to reside within the subunit-subunit interface of the Rad51 filament. To study the function of the Tyr315 residue, we purified five Rad51 mutants, Y315D, Y315E, Y315R, Y315A and Y315F, in which the Tyr315 residue was replaced by Asp, Glu, Arg, Ala and Phe, respectively. Biochemical studies of these Rad51 mutants revealed that the Y315D and Y315E mutants are defective in homologous pairing due to their impaired ssDNA binding, but their dsDNA binding remained unaffected. The Y315D, Y315E and Y315R mutants are defective in dsDNA unwinding, which depends on Rad51-filament formation, suggesting that these mutants are defective in filament formation on dsDNA. Therefore, the Rad51-Tyr315 residue plays important roles in ssDNA binding and filament formation.

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Citations

Dec 14, 2011·Nucleic Acids Research·Yuichi MorozumiHitoshi Kurumizaka
Aug 2, 2007·Biological & Pharmaceutical Bulletin·Takako IshidaHitoshi Kurumizaka
Jan 5, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Takako IshidaHitoshi Kurumizaka
Apr 24, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Julian NommeMasayuki Takahashi
Mar 8, 2011·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Motoki TakakuHitoshi Kurumizaka
Jun 9, 2015·Chemistry & Biology·Sylvain ThierryVincent Parissi
Mar 17, 2009·Biochemical and Biophysical Research Communications·Hiroko ShimizuKen-ichi Yamamoto
Aug 16, 2014·Biochemical Pharmacology·Anaïs NormandAxelle Renodon-Cornière
Sep 28, 2016·Proceedings of the National Academy of Sciences of the United States of America·Shyamal SubramanyamMaria Spies
Sep 14, 2019·Veterinary and Comparative Oncology·Mitsuki UemuraToshinori Omi
Jul 18, 2008·Cancer Research·Suganthini S GanapathipillaiYitzhak Zimmer
Oct 8, 2021·Frontiers in Oncology·Arafat SiddiquiSakari Hietanen

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