Mutational analysis by next generation sequencing of gastric type dysplasia occurring in hyperplastic polyps of the stomach: Mutations in gastric hyperplastic polyps

Experimental and Molecular Pathology
Marcela SalomaoAntonia R Sepulveda

Abstract

Gastric hyperplastic polyps (GHP) are the most common type of polyps occurring in the stomach. Although GHP are broadly interpreted as benign lesions, they may progress to dysplasia and adenocarcinoma. In this study, we aimed to identify genomic mutations that characterize and may drive malignant transformation in GHP by using next-generation sequencing. Eight GHP (2 with dysplasia, 1 indefinite for dysplasia and 5 without dysplasia) were studied. Only large polyps (>1cm) with gastric differentiation were included in this study, while adenomatous polyps (intestinal-type) were excluded. Immunohistochemistry for MUC2, MUC5A, MUC6, CDX2, p53, and Ki67 was performed. DNA was extracted from formalin-fixed paraffin-embedded sections and sequenced for the detection of somatic mutations. Multiplex sequencing was done with the TrueSeq Amplicon Cancer Panel in the MiSeq platform. Variant annotation and visualization were performed using NextGENe (SoftGenetics) software. No pathogenic mutations were detected in GHP without dysplasia. TP53 gene mutations were the most common alteration in dysplastic GHP (2 of 2 dysplastic cases). PIK3CA mutation was identified in a GHP with pyloric-type dysplasia, whereas foveolar-type dysplasia carried TP...Continue Reading

References

Feb 1, 1996·Digestive Diseases and Sciences·W L Zea-IriarteT Hattori
Mar 16, 2004·Science·Yardena SamuelsVictor E Velculescu
Jul 5, 2005·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Sérgia VelhoRaquel Seruca
Jul 6, 2011·Nucleic Acids Research·Boris RevaChris Sander
Oct 12, 2012·PloS One·Yongwook ChoiAgnes P Chan
Aug 1, 2014·Nature·UNKNOWN Cancer Genome Atlas Research Network

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Citations

Jan 14, 2017·Der Pathologe·C Röcken
Feb 2, 2017·International Journal of Molecular Sciences·Rick KampsAndrea Romano
Jun 30, 2019·Virchows Archiv : an International Journal of Pathology·Yoshiyasu TakayamaYasuhiro Onozato
Jun 14, 2020·Virchows Archiv : an International Journal of Pathology·Tamotsu SugaiTakayuki Matsumoto
Jan 1, 2021·Histopathology·Bence KőváriGregory Y Lauwers
Jun 3, 2021·Journal of Clinical Medicine·Seung Woo LeeJoonhong Park
Jun 18, 2021·Indian Journal of Pathology & Microbiology·Anjali D AmarapurkarAkash P Shukla

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