PMID: 9431454Feb 12, 1998Paper

Mutational analysis of Csc1/Vps4p: involvement of endosome in regulation of autophagy in yeast

Cell Structure and Function
K ShirahamaYoshinori Ohsumi

Abstract

In the yeast Saccharomyces cerevisiae, autophagy, a bulk protein degradation in the vacuole, is induced in response to nutrient starvation. In a screen for mutations that result in induction of autophagy even in the presence of nutrients, we have isolated four mutants representing two csc complementation groups. These mutants induce autophagy of which activity is represented by activation of truncated alkaline phosphatase that is designed to be expressed in the cytosol. CSC1 was cloned by complementation of loss of viability phenotype of csc1-1 mutant and shown to be identical to END13/VPS4/GRD13. Though csc1-1 mutation is recessive, cells of delta csc1 do not induce autophagy in rich media, suggesting that csc1-1 allele is not a complete loss-of-function. Csc1p is a member of novel ATPase family named AAA protein including Sec18p/NSF, Cdc48p/p97, and Pas8p. Mutation site in csc1-1 is found in the SRH region that is highly conserved among AAA proteins. Cells of csc1-1 show sorting defect of CPY and the appearance of the class E compartment. These mutant phenotypes suggest the role of the protein that is involved in the traffic among the Golgi, endosome, and the vacuole in autophagy.

Citations

Nov 29, 2002·Eukaryotic Cell·Fulvio Reggiori, Daniel J Klionsky
Jan 5, 2011·Antioxidants & Redox Signaling·Tomotake KankiKoji Okamoto
Jun 16, 2010·Cell Research·Maryam MehrpourPatrice Codogno
Dec 28, 1999·Annual Review of Cell and Developmental Biology·D J Klionsky, Y Ohsumi
Oct 20, 1999·The Journal of Cell Biology·T KirisakoY Ohsumi
May 11, 2007·Biotechnology Advances·Eduardo Cebollero, Ramon Gonzalez
Sep 3, 2009·Biotechnology Progress·Eduardo CebolleroRamon Gonzalez
Jul 29, 2010·The Plant Journal : for Cell and Molecular Biology·Mojgan ShahriariSwen Schellmann
Aug 31, 2000·Annual Review of Biochemistry·J Kim, D J Klionsky
Jul 23, 2008·Biochimica Et Biophysica Acta·Ashley R Winslow, David C Rubinsztein
May 26, 2005·Biochimica Et Biophysica Acta·Katherine Bowers, Tom H Stevens
Nov 23, 2017·Journal of Experimental Botany·Kamila Kalinowska, Erika Isono
Apr 9, 2002·Cell Structure and Function·Atsuki NaraTamotsu Yoshimori
Feb 25, 2006·The Journal of Biological Chemistry·Sébastien BesteiroJeremy C Mottram
Apr 8, 2006·Physiological Reviews·Olivier Staub, Daniela Rotin
Jan 5, 2011·Protein & Cell·Jingjing TongLi Yu
Oct 21, 2010·Physiological Reviews·Brinda RavikumarDavid C Rubinsztein
Nov 28, 2002·Traffic·Eeva-Liisa EskelinenJohn M Lucocq
Aug 2, 2019·Autophagy·Yan ZhenHarald Stenmark
Jun 19, 2009·Journal of Cell Science·Tor Erik Rusten, Harald Stenmark
Aug 28, 1998·Current Opinion in Cell Biology·S V Scott, D J Klionsky
Aug 28, 1998·Current Opinion in Cell Biology·M A De Matteis, J S Morrow

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