Mutational analysis of Deinococcus radiodurans bacteriophytochrome reveals key amino acids necessary for the photochromicity and proton exchange cycle of phytochromes

The Journal of Biological Chemistry
Jeremiah R WagnerRichard D Vierstra

Abstract

The ability of phytochromes (Phy) to act as photointerconvertible light switches in plants and microorganisms depends on key interactions between the bilin chromophore and the apoprotein that promote bilin attachment and photointerconversion between the spectrally distinct red light-absorbing Pr conformer and far red light-absorbing Pfr conformer. Using structurally guided site-directed mutagenesis combined with several spectroscopic methods, we examined the roles of conserved amino acids within the bilin-binding domain of Deinococcus radiodurans bacteriophytochrome with respect to chromophore ligation and Pr/Pfr photoconversion. Incorporation of biliverdin IXalpha (BV), its structure in the Pr state, and its ability to photoisomerize to the first photocycle intermediate are insensitive to most single mutations, implying that these properties are robust with respect to small structural/electrostatic alterations in the binding pocket. In contrast, photoconversion to Pfr is highly sensitive to the chromophore environment. Many of the variants form spectrally bleached Meta-type intermediates in red light that do not relax to Pfr. Particularly important are Asp-207 and His-260, which are invariant within the Phy superfamily and par...Continue Reading

References

Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·K C Yeh, J C Lagarias
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·H HanzawaM Furuya
Feb 12, 2002·Nature Reviews. Molecular Cell Biology·Peter H Quail
Nov 19, 2003·Biochemistry·Berthold BoruckiTilman Lamparter
Nov 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·Amanda J Fischer, J Clark Lagarias
Dec 22, 2004·Accounts of Chemical Research·Julie M StevensStuart J Ferguson
Dec 23, 2004·Journal of the American Chemical Society·Maria Andrea MroginskiPeter Hildebrandt
Aug 3, 2005·The Journal of Biological Chemistry·Berthold BoruckiPeter Hildebrandt
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·W RüdigerS Schneider
May 4, 2006·Annual Review of Plant Biology·Nathan C RockwellJ Clark Lagarias
May 13, 2006·The FEBS Journal·Janina HahnJon Hughes
Jun 29, 2006·The Journal of Biological Chemistry·Katsuhiko InomataTilman Lamparter
Feb 27, 2007·The Journal of Biological Chemistry·Jeremiah R WagnerKatrina T Forest
Jul 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Xiaojing YangKeith Moffat

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Citations

Jan 5, 2011·The Journal of Physical Chemistry. a·K C TohJohn T M Kennis
Nov 5, 2010·The Journal of Physical Chemistry. B·Alex A SammaMarc Zimmer
Jan 16, 2010·Nature·Andrew T UlijaszRichard D Vierstra
Jul 19, 2011·Nature Biotechnology·Grigory S FilonovVladislav V Verkhusha
Jul 12, 2013·Nature Communications·Kiryl D PiatkevichVladislav V Verkhusha
Sep 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Xiaojing YangKeith Moffat
Sep 2, 2009·Proceedings of the National Academy of Sciences of the United States of America·Xiaojing YangKeith Moffat
Apr 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Nathan C RockwellJ Clark Lagarias
May 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·K C TohJohn T M Kennis
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Hua LiHuilin Li
Feb 18, 2011·Proceedings of the National Academy of Sciences of the United States of America·Chen SongJörg Matysik
Nov 13, 2010·The Journal of Biological Chemistry·Benjamin ZienickeTilman Lamparter
Jan 3, 2012·The Journal of Biological Chemistry·Michele E AuldridgeKatrina T Forest
Oct 22, 2008·The Journal of Biological Chemistry·Sonja BrandtNicole Frankenberg-Dinkel
Sep 6, 2008·Genome Biology·Robert A Sharrock
Dec 18, 2013·Biochemistry·Francisco Velazquez EscobarPeter Hildebrandt
Apr 30, 2014·Nature·Heikki TakalaSebastian Westenhoff
Feb 26, 2014·Proceedings of the National Academy of Sciences of the United States of America·Nathan C RockwellJ Clark Lagarias
Jan 1, 2014·Proceedings of the National Academy of Sciences of the United States of America·Kathryn R FixenCaroline S Harwood
Jun 4, 2014·Proceedings of the National Academy of Sciences of the United States of America·Carlos GasserAndreas Möglich
Jul 2, 2014·Proceedings of the National Academy of Sciences of the United States of America·E Sethe BurgieRichard D Vierstra
Mar 3, 2010·Annual Review of Plant Biology·Andreas MöglichKeith Moffat
Jan 31, 2013·Chemical Society Reviews·Kiryl D PiatkevichVladislav V Verkhusha
Apr 23, 2013·The Journal of Biological Chemistry·Johannes SalewskiPeter Hildebrandt
Jul 2, 2011·Trends in Plant Science·Richard D Vierstra, Junrui Zhang
Jan 19, 2016·Frontiers in Molecular Biosciences·Janne A IhalainenHeli Lehtivuori
Jul 8, 2011·Current Opinion in Plant Biology·Andrew T Ulijasz, Richard D Vierstra
Dec 19, 2015·Scientific Reports·Konstantin A RumyantsevVladislav V Verkhusha
Feb 28, 2014·Photochemistry and Photobiology·Vitaly SineshchekovJon Hughes
Nov 23, 2015·Chemistry & Biology·Daria M ShcherbakovaVladislav V Verkhusha
Aug 16, 2015·The Journal of Physical Chemistry Letters·Alexander BjörlingSebastian Westenhoff
Jun 27, 2015·Current Opinion in Chemical Biology·Daria M ShcherbakovaVladislav V Verkhusha
Aug 19, 2015·Frontiers in Molecular Biosciences·Chen SongJörg Matysik

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