Mutational analysis of functional domains in Mrs2p, the mitochondrial Mg2+ channel protein of Saccharomyces cerevisiae

The FEBS Journal
Julian WeghuberR J Schweyen

Abstract

The nuclear gene MRS2 in Saccharomyces cerevisiae encodes an integral protein (Mrs2p) of the inner mitochondrial membrane. It forms an ion channel mediating influx of Mg2+ into mitochondria. Orthologues of Mrs2p have been shown to exist in other lower eukaryotes, in vertebrates and in plants. Characteristic features of the Mrs2 protein family and the distantly related CorA proteins of bacteria are the presence of two adjacent transmembrane domains near the C terminus of Mrs2p one of which ends with a F/Y-G-M-N motif. Two coiled-coil domains and several conserved primary sequence blocks in the central part of Mrs2p are identified here as additional characteristics of the Mrs2p family. Gain-of-function mutations obtained upon random mutagenesis map to these conserved sequence blocks. They lead to moderate increases in mitochondrial Mg2+ concentrations and concomitant positive effects on splicing of mutant group II intron RNA. Site-directed mutations in several conserved sequences reduce Mrs2p-mediated Mg2+ uptake. Mutants with strong effects on mitochondrial Mg2+ concentrations also have decreased group II intron splicing. Deletion of a nonconserved basic region, previously invoked for interaction with mitochondrial introns, lowe...Continue Reading

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Citations

Jun 3, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Muhammad Bashir KhanKristina Djinović-Carugo
Jun 16, 2007·Biological Chemistry·Olga Fedorova, Nora Zingler
May 21, 2013·Metallomics : Integrated Biometal Science·Gerhard SponderKristina Djinović-Carugo
Aug 22, 2007·Molecular Membrane Biology·Krisztina M Papp-Wallace, Michael E Maguire
Aug 15, 2006·The FEBS Journal·Marcin WachekAnton Graschopf
Jul 27, 2010·The FEBS Journal·Gerhard SponderJulian Weghuber
Sep 11, 2007·Biophysical Journal·Rainer SchindlRudolf J Schweyen
Mar 19, 2014·The Journal of Biological Chemistry·Takeshi HaraguchiKohji Ito
Apr 3, 2014·Physiological Reviews·Ildiko Szabo, Mario Zoratti
Nov 27, 2009·American Journal of Physiology. Cell Physiology·Gary A Quamme
Feb 16, 2008·The Journal of Biological Chemistry·Jian PayandehEmil F Pai

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