Mutational analysis of interactions between the Gag precursor proteins of murine leukemia viruses

Virology
K Alin, S P Goff

Abstract

The yeast two-hybrid system was used to test for interactions among the Gag precursor proteins of three members of the murine leukemia virus family. These Gag proteins all interact with each other in all combinations, but do not interact with the distantly related HIV-1 Gag. A series of deletion mutants of Moloney MuLV were examined to determine the minimal interaction domain. Either one of two regions of Gag was independently sufficient to mediate homodimerization, suggesting multiple points of contact in the precursor. Analysis of a set of point mutations in the CA region revealed a complex pattern of effects on Gag-Gag interactions.

Citations

Feb 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yuhe YanChristine A Kozak
Jul 20, 2007·Journal of Virology·Samuel L Murphy, Glen N Gaulton
Jun 22, 2012·Journal of Virology·Lisa GreenStephen P Goff
Jan 18, 2006·Journal of Virology·William FuWei-Shau Hu
Dec 9, 2003·Journal of Biology·Margaret Q WangStephen P Goff
May 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Elena CasacubertaMary-Lou Pardue
Nov 13, 1999·Journal of Virology·Y TangH C Morse
Nov 1, 1996·Journal of Virology·Q K ZhangS P Goff

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