Mutational analysis of intervening sequences connecting the binding sites for integration host factor, PepA, PurR, and RNA polymerase in the control region of the Escherichia coli carAB operon, encoding carbamoylphosphate synthase.

Journal of Bacteriology
Neel DevroedeDaniel Charlier

Abstract

Transcription of the carAB operon encoding the unique carbamoylphosphate synthase of Escherichia coli reflects the dual function of carbamoylphosphate in the biosynthesis of arginine and pyrimidine nucleotides. The tandem pair of promoters is regulated by various mechanisms depending on the needs of both pathways and the maintenance of a pyrimidine/purine nucleotide balance. Here we focus on the linker regions that impose the distribution of target sites for DNA-binding proteins involved in pyrimidine- and purine-specific repression of the upstream promoter P1. We introduced deletions and insertions, and combinations thereof, in four linkers connecting the binding sites for integration host factor (IHF), PepA, PurR, and RNA polymerase and studied the importance of phasing and spacing of the targets and the importance of the nucleotide sequence of the linkers. The two PepA binding sites must be properly aligned and separated with respect to each other and to the promoter for both pyrimidine- and purine-mediated repression. Similarly, the phasing and spacing of the IHF and PEPA2 sites are strictly constrained but only for pyrimidine-specific repression. The IHF target is even dispensable for purine-mediated regulation. Thus, a co...Continue Reading

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Citations

Jan 17, 2012·Applied Microbiology and Biotechnology·Yung-Hun YangByung-Gee Kim
Apr 23, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jana StöckelHimadri B Pakrasi
May 18, 2010·Nucleic Acids Research·Phu Le Minh NguyenDaniel Charlier
Jul 1, 2009·Infection and Immunity·Basel H Abuaita, Jeffrey H Withey
Jun 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Charles L Turnbough, Robert L Switzer
Oct 29, 2008·Genome Biology·Julio A Freyre-GonzálezJulio Collado-Vides
May 17, 2011·The New Phytologist·Rungaroon Waditee-SirisatthaMasafumi Tsujimoto
Jul 8, 2015·Microbial Cell Factories·Jin-Sook KimHyeon-Cheol Lee
Sep 16, 2011·Plant Signaling & Behavior·Rungaroon Waditee-SirisatthaMasafumi Tsujimoto
Sep 1, 2008·EcoSal Plus·Kaj Frank JensenMartin WillemoËs

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