Mutational analysis of putative helix-helix interacting GxxxG-motifs and tryptophan residues in the two-peptide bacteriocin lactococcin G

Biochemistry
Camilla OppegårdJ Nissen-Meyer

Abstract

The membrane-permeabilizing two-peptide bacteriocin lactococcin G consists of two different peptides, LcnG-alpha and LcnG-beta. The bacteriocin contains several tryptophan and tyrosine residues and three putative helix-helix interacting GxxxG-motifs, G 7xxxG 11 and G 18xxxG 22 in LcnG-alpha and G 18xxxG 22 in LcnG-beta. The tryptophan and tyrosine residues and residues in the GxxxG-motifs were altered by site-directed mutagenesis to analyze the structure and membrane-orientation of lactococcin G. Substituting the glycine residues at position 7 or 11 in the G 7xxxG 11-motif in LcnG-alpha with large hydrophobic or hydrophilic residues was highly detrimental, whereas small residues were tolerated. Qualitatively similar results were obtained for the G 18xxxG 22-motif in LcnG-beta. In contrast, replacement of the glycine residues in the middle of these two motifs with large hydrophilic residues was tolerated. All mutations in the G 18xxxG 22-motif in LcnG-alpha were relatively well-tolerated, indicating that this motif is not involved in helix-helix interactions. The four aromatic residues in the N-terminal part of LcnG-beta could individually be replaced by other aromatic residues, a hydrophilic positive residue, and a hydrophobic ...Continue Reading

References

Feb 1, 1991·Applied and Environmental Microbiology·M J van BelkumG Venema
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Nov 1, 1993·Applied and Environmental Microbiology·C FremauxT R Klaenhammer
Feb 1, 1996·Journal of Bacteriology·G MollA J Driessen
Jan 9, 1998·Journal of Bacteriology·G MollA J Driessen
Oct 21, 1998·Biochemistry·W M YauS H White
Nov 13, 1998·FEMS Microbiology Letters·L AxelssonA Holck
Jul 20, 1999·The Journal of Biological Chemistry·M R de PlanqueJ A Killian
Sep 6, 2000·Trends in Biochemical Sciences·J A Killian, G von Heijne
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·A SenesD M Engelman
Apr 27, 2002·Applied and Environmental Microbiology·Charles M A P FranzWilhelm H Holzapfel
Jul 24, 2002·Biochemistry·Gunnar FimlandJon Nissen-Meyer
Feb 5, 2003·Current Microbiology·Sergio A CuozzoRaul R Raya
Jun 18, 2003·International Journal of Food Microbiology·Patricia CastellanoGraciela Vignolo
Aug 18, 2004·Current Opinion in Structural Biology·Alessandro SenesWilliam F DeGrado
Oct 6, 2005·Nature Reviews. Microbiology·Paul D CotterR Paul Ross
May 5, 2006·Applied and Environmental Microbiology·Takeshi ZendoKenji Sonomoto
Feb 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Dzung B DiepIngolf F Nes
Mar 6, 2007·Applied and Environmental Microbiology·Camilla OppegårdJon Nissen-Meyer
Sep 11, 2007·Journal of Molecular Microbiology and Biotechnology·Camilla OppegårdJon Nissen-Meyer
Jan 12, 2008·Biochimica Et Biophysica Acta·Per RognePer Eugen Kristiansen

❮ Previous
Next ❯

Citations

Jul 21, 2010·Applied Microbiology and Biotechnology·Takeshi ZendoKenji Sonomoto
Jan 14, 2010·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·J Dennis PollackDennis K Pearl
Jun 29, 2010·Applied and Environmental Microbiology·Eileen F O'SheaColin Hill
Sep 14, 2010·Applied and Environmental Microbiology·Rubén CebriánManuel Montalbán-López
Jan 18, 2016·Biochimica Et Biophysica Acta·Panagiota K KyriakouYiannis N Kaznessis
Aug 23, 2011·FEBS Letters·Angélica OspinaJosé A Carrodeguas
Jun 23, 2009·Peptides·Per RognePer Eugen Kristiansen
Aug 20, 2016·Biochemistry·Bie EkbladPer Eugen Kristiansen
Apr 13, 2010·Probiotics and Antimicrobial Proteins·Jon Nissen-MeyerPer Eugen Kristiansen
Aug 8, 2018·FEMS Microbiology Reviews·Jeella Z AcedoMarco J van Belkum
Aug 18, 2020·Applied and Environmental Microbiology·Trine NilsenThomas P Parks
Oct 17, 2017·Scientific Reports·Jonathan ZerweckAnne S Ulrich

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.