Mutational Analysis of the Bovine Hepacivirus Internal Ribosome Entry Site

Journal of Virology
Anna Lena BaronChristine Baechlein

Abstract

In recent years, hepatitis C virus (HCV)-related viruses were identified in several species, including dogs, horses, bats, and rodents. In addition, a novel virus of the genus Hepacivirus has been discovered in bovine samples and was termed bovine hepacivirus (BovHepV). Prediction of the BovHepV internal ribosome entry site (IRES) structure revealed strong similarities to the HCV IRES structure comprising domains II, IIIabcde, pseudoknot IIIf, and IV with the initiation codon AUG. Unlike HCV, only one microRNA-122 (miR-122) binding site could be identified in the BovHepV 5' nontranslated region. In this study, we analyzed the necessity of BovHepV IRES domains to initiate translation and investigated possible interactions between the IRES and core coding sequences by using a dual luciferase reporter assay. Our results suggest that such long-range interactions within the viral genome can affect IRES-driven translation. Moreover, the significance of a possible miR-122 binding to the BovHepV IRES was investigated. When analyzing translation in human Huh-7 cells with large amounts of endogenous miR-122, introduction of point mutations to the miR-122 binding site resulted in reduced translation efficiency. Similar results were observ...Continue Reading

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Citations

May 7, 2020·Nucleic Acids Research·Konstantinos G KokkonosTroels K H Scheel
Aug 22, 2018·Viruses·Annie Bernier, Selena M Sagan
Aug 14, 2020·International Journal of Molecular Sciences·Rasika D KundenJoyce A Wilson

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