PMID: 9525653Apr 3, 1998Paper

Mutational analysis of the candidate internal fusion peptide of the avian leukosis and sarcoma virus subgroup A envelope glycoprotein

Journal of Virology
L D Hernandez, J M White

Abstract

The transmembrane subunit (TM) of the avian leukosis and sarcoma virus (ALSV) envelope glycoprotein (Env) contains a stretch of conserved hydrophobic amino acids internal to its amino terminus (residues 21 to 42). By analogy with similar sequences in other viral envelope glycoproteins, this region has been proposed to be a fusion peptide. We investigated the role of this region by changing each of three hydrophobic residues (Ile-21, Val-30, and Ile-39) to glutamatic acid and lysine in the ALSV subgroup A Env. Like wild-type (wt) Env, all six mutant Env proteins were proteolytically processed, oligomerized, and expressed at the cell surface in a form that bound Tva, the ALSV subgroup A receptor. Like wt Env, Ile21Glu, Ile21Lys, Va30Glu, and Val30Lys changed conformation upon binding Tva, as assayed by sensitivity to thermolysin. Ile39Glu and Ile39Lys were cleaved by thermolysin in both the absence and presence of Tva. Although incorporated into virus particles at approximately equal levels, all mutant Envs were compromised in their ability to support infection. The mutants at residues 21 and 30 showed levels of infection 2 to 3 orders of magnitude lower than that of wt Env. The mutants at residue 39 were noninfectious. Furthermo...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G Sutter, B Moss
Nov 6, 1992·Science·J M White
Aug 1, 1991·Journal of Virology·P Levy-Mintz, M Kielian
Oct 1, 1990·Journal of Virology·M A WhittJ K Rose
Oct 1, 1990·Journal of Virology·J M GilbertJ M White
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E O FreedR Risser
Jan 1, 1989·Advances in Virus Research·M Marsh, A Helenius
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D Einfeld, E Hunter
Jan 1, 1987·Methods in Enzymology·T A KunkelR A Zakour
Jan 1, 1984·Annual Review of Biochemistry·D Eisenberg
Nov 1, 1993·Virology·R A Lamb
Jan 1, 1995·Cold Spring Harbor Symposia on Quantitative Biology·J J SkehelD C Wiley
Jan 1, 1996·Annual Review of Cell and Developmental Biology·L D HernandezJ M White

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Citations

Dec 21, 2004·European Journal of Biochemistry·Shu-Fang ChengDing-Kwo Chang
Feb 12, 2008·PLoS Pathogens·Jason G Smith, James M Cunningham
Mar 29, 2014·The Journal of Biological Chemistry·Yan LiJaume Torres
Mar 5, 2016·Traffic·Judith M White, Gary R Whittaker
Mar 22, 2002·Journal of Virology·Karin StiasnyFranz X Heinz
Jun 24, 2008·Critical Reviews in Biochemistry and Molecular Biology·Judith M WhiteKathryn Schornberg
Jul 23, 2003·Biochimica Et Biophysica Acta·Richard M Epand

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