Mutational analysis of the cell cycle inhibitor Kip1/p27 in childhood leukemia

Pediatric Blood & Cancer
E-A MarkakiM Kalmanti

Abstract

Cyclin-dependent kinases (CDKs) and cyclins, their regulatory subunits, govern cell-cycle progression in eukaryotic cells. Kip1/p27 is the main cyclin-dependent kinase inhibitor, which arrests cell division inhibiting G1-S transition. Kip1/p27 seems to play a critical role in the pathogenesis of several human malignancies and its lower expression has been shown to correlate with a poor prognosis in adult solid tumors. Bone marrow blasts from 49 children with leukemia, 37 acute lymphoblastic leukemia (ALL), and 12 acute myeloid leukemia (AML) were studied. Exon 3 of Kip1/p27 was amplified using the polymerase chain reaction technique (PCR). Single strand conformational polymorphism and heterodouplex analysis were performed to detect DNA sequence with altered conformations and were subsequently sequenced to document mutations. Mutations in Kip1/p27 gene were detected in 2 out of 3 T-ALL, 6 out of 12 AML patients, and only 1 out of 34 B lineage ALL cases. Although the patient groups are small, a highly significant relation of the mutation status with the type of leukemia (P = 0.0037) and the risk group according to treatment protocols (P = 0.00021) was estimated. A statistically significant difference in the white blood count was ...Continue Reading

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Citations

Apr 25, 2012·Current Hematologic Malignancy Reports·Ilaria IacobucciGiovanni Martinelli
Feb 4, 2011·The Journal of Clinical Endocrinology and Metabolism·Jessica Costa-GudaAndrew Arnold
Aug 24, 2011·Leukemia & Lymphoma·Bettina R BonnBirgit Burkhardt
Sep 11, 2014·Journal of Cellular Physiology·Anita Roy, Subrata Banerjee
Dec 8, 2009·Cancer Genetics and Cytogenetics·Aleksandra Drago KrsticClelia Tiziana Storlazzi
Jun 19, 2015·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Ansar HakeemA R Lone
Feb 9, 2010·Neurology India·Usha Kant Misra, Jayantee Kalita
Jun 15, 2011·Journal of Cell Science·Akihiko YokoyamaMichael L Cleary

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