PMID: 2120472Nov 1, 1990Paper

Mutational analysis of the human immunodeficiency virus type 1 Rev transactivator: essential residues near the amino terminus

Journal of Virology
T J HopeT G Parslow

Abstract

The expression of certain mRNAs from human immunodeficiency virus type 1 (HIV-1) is controlled by the viral transactivator Rev, a nucleolar protein that binds a cis-acting element in these mRNAs. Rev is encoded by two viral exons that specify amino acids 1 to 26 and 27 to 116, respectively. Earlier studies have mapped essential regions of the protein that are encoded in the second exon. By further mutational analysis of Rev, we have now identified a novel locus encoded by the first exon that also is essential for transactivation in vivo. Defined by mutations at residues 14 to 20, this locus coincides with a cluster of positively charged and nonpolar amino acids that is conserved in Rev proteins of all known primate immunodeficiency viruses. Rev proteins that contained mutations at this site were defective in both nuclear localization and transactivation and did not function as trans-dominant inhibitors of wild-type Rev. Fusion of these mutants to a heterologous nuclear protein complemented the defect in localization but did not restore biological activity. Our findings suggest that this N-terminal locus may play a direct role in transactivation, perhaps contributing to essential protein-protein interactions or forming part of t...Continue Reading

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Citations

Oct 19, 2002·Journal of Biomedical Science·M.J. Zhang, A.I. Dayton
Aug 6, 2004·The Journal of Biological Chemistry·Dirk DaelemansGeorge N Pavlakis
Aug 29, 2006·Nucleic Acids Research·Kuan-Teh Jeang, Venkat Yedavalli
Jul 21, 2011·Retrovirology·Venkat S R K Yedavalli, Kuan-Teh Jeang
Mar 9, 2002·Molecular and Cellular Biology·Ileana PopaThomas J Hope
Nov 8, 2002·American Journal of Pharmacogenomics : Genomics-related Research in Drug Development and Clinical Practice·Franco LoriJulianna Lisziewicz
Nov 1, 2000·The Journal of Biological Chemistry·W J Friesen, M K Darby
Jan 10, 2002·The Journal of Biological Chemistry·Bin Li, T S Benedict Yen
Aug 9, 2002·AIDS Research and Human Retroviruses·Jianhua FangRoger J Pomerantz
Apr 16, 2005·The Journal of General Virology·Roochi Trikha, David W Brighty
Nov 11, 2009·PloS One·Luke W MeredithDavid Harrich
Aug 8, 2015·Viruses·Bárbara Rojas-ArayaRicardo Soto-Rifo
Jun 30, 2016·Biopolymers·Santosh Kumar Upadhyay
Jan 19, 1999·Annual Review of Microbiology·V W Pollard, M H Malim
Dec 31, 1997·The Journal of Experimental Medicine·C B DavisD R Littman
Dec 5, 1998·The Journal of Biological Chemistry·P AskjaerJ Kjems
Apr 29, 1999·Nucleic Acids Research·P C BriceP J Butler
Apr 12, 1996·The Journal of Biological Chemistry·J J Hunter, T G Parslow
May 18, 2006·The Journal of Biological Chemistry·Marc ArnoldRalph H Kehlenbach
Mar 27, 2009·The Journal of General Virology·H C T GroomA M L Lever
Jan 26, 2008·Protein Science : a Publication of the Protein Society·Stephen P EdgcombMirko Hennig

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