PMID: 9445027Jan 28, 1998Paper

Mutational analysis of the human immunodeficiency virus type 1 Vpu transmembrane domain that promotes the enhanced release of virus-like particles from the plasma membrane of mammalian cells

Journal of Virology
M PaulM A Jabbar

Abstract

Human immunodeficiency virus type 1 Vpu is a multifunctional phosphoprotein composed of the N-terminal transmembrane (VpuTM) and C-terminal cytoplasmic domains. Each of these domains regulates a distinct function of the protein; the transmembrane domain is critical in virus release, and phosphorylation of the cytoplasmic domain is necessary for CD4 proteolysis. We carried our experiments to identify amino acids in the VpuTM domain that are important in the process of virus-like particle (VLP) release from HeLa cells. VLPs are released from the plasma membrane of HeLa cells at constitutive levels, and Vpu expression enhanced the release of VLPs by a factor of 10 to 15. Deletion of two to five amino acids from both N- and C-terminal ends or the middle of the VpuTM domain generated mutant Vpu proteins that have lost the ability to enhance VLP release. These deletion mutants have not lost the ability to associate with the wild-type or mutant Vpu proteins and formed complexes with equal efficiency. They were also transported normally to the Golgi complex. Furthermore, a Vpu protein having the CD4 transmembrane and Vpu cytoplasmic domains was completely inactive, and Vpu proteins harboring hybrid Vpu-CD4 TM domains were also defectiv...Continue Reading

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Citations

Nov 24, 1999·The Journal of Biological Chemistry·S BourK Strebel
Feb 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gangadhara SailajaAbdul M Jabbar
Feb 22, 2003·Journal of Biomedical Science·Yi-Ming A ChenKwang-Jen Hsiao
Sep 18, 2009·BMC Medicine·Klaus StrebelKuan-Teh Jeang
Apr 5, 2016·Immunity, Inflammation and Disease·Wadie D Mahauad-Fernandez, Chioma M Okeoma
Mar 29, 2014·Biopolymers·Zeyun MiShan Cen
Oct 6, 2010·Virology Journal·Cristian De CandiaMauricio Carobene

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