PMID: 7543592Sep 1, 1995Paper

Mutational analysis of the interaction between the bovine papillomavirus E5 transforming protein and the endogenous beta receptor for platelet-derived growth factor in mouse C127 cells

Journal of Virology
L A NilsonD DiMaio

Abstract

The bovine papillomavirus E5 protein is a 44-amino-acid membrane-associated protein that forms a stable complex with the endogenous platelet-derived growth factor (PDGF) beta receptor in rodent and bovine fibroblasts, resulting in sustained receptor activation and cell transformation. We report here that high-level expression of the E5 protein caused a reduction in the level of the mature form of the PDGF beta receptor in acutely and stably transformed mouse C127 cells. To explore in more detail the interaction of the E5 protein and the PDGF beta receptor, we tested the abilities of various E5 point mutants to bind the PDGF receptor, to induce PDGF receptor down-regulation and tyrosine phosphorylation, and to transform cells. A transformation-competent mutant, like the wild-type E5 protein, bound the receptor and induced receptor tyrosine phosphorylation and down-regulation. Transformation-defective E5 proteins either failed to interact with the endogenous PDGF beta receptor in mouse fibroblasts or underwent an aberrant interaction with the receptor. Mutation of glutamine at position 17, aspartic acid at position 33, or both carboxyl-terminal cysteine residues required for E5 homodimerization interfered with stable complex form...Continue Reading

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S BurnettD DiMaio
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·L Petti, D DiMaio
Jan 25, 1991·Cell·L C CantleyS Soltoff
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J Settleman, D DiMaio
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D DiMaioJ T Schiller
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J NilssonA Wasteson
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·A N MeyerD J Donoghue

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Citations

Dec 26, 2001·Oncogene·D DiMaio, D Mattoon
May 16, 2008·Veterinary Research·Giuseppe Borzacchiello, Franco Roperto
Aug 12, 1999·Annals of Medicine·S M Syrjänen, K J Syrjänen
Dec 28, 2019·The Journal of Biological Chemistry·Justin M Westerfield, Francisco N Barrera
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·C C LaiD DiMaio
Dec 15, 2010·Protein Engineering, Design & Selection : PEDS·Sara A MarlattDaniel Dimaio
Feb 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Tobin J CammettDaniel Dimaio
Aug 16, 2017·Proceedings of the National Academy of Sciences of the United States of America·Alexander G KarabadzhakDaniel DiMaio
Apr 20, 2001·The International Journal of Biochemistry & Cell Biology·U VäliM Ustav
Jun 1, 1996·Journal of Virology·S L ChenY P Tsao
Jun 25, 2003·Protein Expression and Purification·Dan-Hui YangFrances J Sharom

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