Mutational analysis of the oxidoreductase ERp57 reveals the importance of the two central residues in the redox motif

FEBS Letters
Siân M Beynon-JonesSimon J Powis

Abstract

The oxidoreductase ERp57 is involved in the formation and breaking of disulfide bonds in assembling proteins within the environment of the endoplasmic reticulum. Site-directed mutants of the redox-active Cys-Gly-His-Cys motif within an isolated ERp57 sub-domain have been studied. Whereas mutation of either cysteine residue abolished reductase activity, substitution of the central residues resulted in retention of partial activity. Alkylation studies indicated that the central residue mutants retained the normal disulfide bond in the motif, whereas this disulfide bond became more resistant to reduction following addition of a third residue into the redox motif, demonstrating an optimum spacing within the redox-active motif of ERp57.

References

Dec 15, 1995·Cell·U GrauschopfJ C Bardwell
Dec 1, 1995·Protein Science : a Publication of the Protein Society·J KemminkT E Creighton
Jun 18, 1998·Folding & Design·M Huber-Wunderlich, R Glockshuber
Jun 25, 1998·Current Biology : CB·E A Hughes, P Cresswell
Jun 25, 1998·Current Biology : CB·N A Morrice, S J Powis
Feb 3, 1999·Annual Review of Genetics·A Rietsch, J Beckwith
Mar 23, 1999·The Biochemical Journal·D M Ferrari, H D Söling
Feb 7, 2001·The Journal of Cell Biology·P NørgaardJ R Winther
Sep 19, 2003·Antioxidants & Redox Signaling·Antony N Antoniou, Simon J Powis
Dec 9, 2003·Journal of Molecular Biology·A K LappiL W Ruddock
Dec 20, 2003·The Journal of Biological Chemistry·Antony N AntoniouSimon J Powis
Feb 12, 2004·The Journal of Biological Chemistry·Eva-Maria FrickelLars Ellgaard
Oct 28, 2004·The Journal of Biological Chemistry·Catherine E Jessop, Neil J Bulleid

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Citations

Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yuichiro ShimizuLinda M Hendershot
Feb 16, 2011·Molecular and Cellular Biology·Iliana Ramírez-RangelJosé Vázquez-Prado
Jul 14, 2010·Cellular and Molecular Life Sciences : CMLS·Emilia PedoneSimonetta Bartolucci
Oct 30, 2007·Biochemical and Biophysical Research Communications·Yoshiko TokutomiShokei Kim-Mitsuyama
Sep 13, 2013·Toxicology Mechanisms and Methods·Xiuzhen FanM Firoze Khan

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