PMID: 9438996Jan 24, 1998Paper

Mutational analysis of the potato virus Y 5' untranslated region for alteration in translational enhancement in tobacco protoplasts

Bioscience, Biotechnology, and Biochemistry
L J YangT Uozumi

Abstract

The 185 nucleotide 5' untranslated region (5'UTR) of potato virus Y ordinary strain (PVY-O) showed translation-enhancing activity on the beta-glucuronidase (GUS) gene in tobacco protoplasts. Mutational analysis of the 5'UTR was done to find sequence motifs necessary for the enhancement. Deletions within the 1-130 nucleotide region of 5'UTR stimulated the GUS expression in some cases, while the GUS activity declined with deletions in the 131-185 nucleotide region. The results indicated that the last 55 nucleotides of PVY-O 5'UTR might play the much important role in the translational enhancement in plant cells.

Citations

Feb 17, 2015·Virus Research·Jincan ZhangAurélie M Rakotondrafara
Oct 2, 2015·Journal of Virology·Robyn RobertsAurélie Rakotondrafara
Apr 22, 2017·Frontiers in Plant Science·Manuel MirasMiguel A Aranda
Dec 20, 2005·Virus Research·Elizabeth L Pettit KnellerW Allen Miller

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