Mutational analysis of the potential catalytic residues of the VV G1L metalloproteinase

Virology Journal
Kady M HoneychurchDennis E Hruby

Abstract

The vaccinia virus G1L open-reading frame is predicted to be a metalloproteinase based upon the presence of a conserved zinc-binding motif. Western blot analysis demonstrates G1L undergoes proteolytic processing during the course of infection, although the significance of this event is unknown. In order to determine which amino acid residues are important for G1L activity, a plasmid-borne library of G1L constructs containing mutations in and about the active site was created. Transient expression analysis coupled with a trans complementation assay of a conditionally-lethal mutant virus suggest that, of the mutants, only glutamic acid 120 is non-essential for G1L processing to occur.

References

Feb 1, 1979·Journal of Virology·D E HrubyJ R Kates
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D E HrubyJ R Kates
May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A B Becker, R A Roth
Nov 1, 1990·Virology·S J GoebelE Paoletti
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S M WilhelmG Goldberg
Oct 10, 1986·Nucleic Acids Research·P W FinchP T Emmerson
Mar 21, 1998·Frontiers in Bioscience : a Journal and Virtual Library·D W Grosenbach, D E Hruby
Mar 27, 1999·Nature·S J Li, M Hochstrasser
Jul 7, 1999·Virus Research·K H MartinD E Hruby
Apr 5, 2000·The Journal of Biological Chemistry·K LehtiJ Keski-Oja
Oct 6, 2000·Virology·D W GrosenbachD E Hruby
May 12, 2001·Pathology Oncology Research : POR·A John, G Tuszynski
Nov 1, 2001·Annual Review of Cell and Developmental Biology·M D Sternlicht, Z Werb
Aug 7, 2002·Journal of Virology·Chelsea M ByrdDennis E Hruby
Sep 27, 2003·Journal of Virology·Chelsea M ByrdDennis E Hruby
Jun 30, 2004·Biochemistry·Jennifer D YoderDennis E Hruby
Jul 6, 2004·Journal of Virological Methods·Marika Hedengren-Olcott, Dennis E Hruby
Aug 11, 2004·European Journal of Mass Spectrometry·Tsefang F ChenDennis E Hruby
Aug 28, 2004·Journal of Virology·Marika Hedengren-OlcottDennis E Hruby
Oct 28, 2004·Journal of Virology·Chelsea M ByrdDennis E Hruby

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Citations

May 20, 2017·Archives of Virology·F G G LeiteT Skern

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Methods Mentioned

BETA
X-ray
transfection
protein folding

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