PMID: 9525671Apr 3, 1998Paper

Mutational analysis of the rous sarcoma virus DR posttranscriptional control element

Journal of Virology
R A Ogert, Karen L Beemon

Abstract

The direct repeat (DR) sequences flanking the src gene in Rous sarcoma virus are essential posttranscriptional control elements; at least one copy of this sequence is necessary for cytoplasmic accumulation of unspliced viral RNA. These sequences promote Rev-independent human immunodeficiency virus type 1 expression, suggesting they act as constitutive transport elements (CTEs). To determine which regions of this sequence are critical for CTE function, mutations in the downstream DR were generated and tested in a viral deletion construct lacking src and the upstream DR. Two single-point mutations and three different clustered mutations caused substantial reductions in reverse transcriptase activity, Gag protein levels, and unspliced viral RNA in the cytoplasm. Three conserved regions of the CTE, including nucleotides 8844 to 8847, 8862 to 8864, and 8868 to 8870, were most sensitive to inactivation by mutagenesis.

References

Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B K FelberG N Pavlakis
Nov 1, 1988·Molecular and Cellular Biology·S Arrigo, K Beemon
Jan 1, 1988·Advances in Virus Research·C M Stoltzfus
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Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·M BrayM L Hammarskjöld
Jan 1, 1997·Molecular and Cellular Biology·R K ErnstM L Hammarskjöld

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Citations

Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lisa Z ScheifeleLeslie J Parent
Mar 9, 2002·Journal of Virology·Andrew W DangelKathleen Boris-Lawrie
Jun 11, 1999·Journal of Virology·B L RuisK F Conklin
Jul 29, 2021·Journal of Virology·Clifton L Ricaña, Marc C Johnson

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