Mutational analysis of the S21 pinholin.

Molecular Microbiology
Ting PangRy Young

Abstract

Lambdoid phage 21 has the prototype pinholin-SAR endolysin lysis system, which is widely distributed among phages. Its prototype pinholin, S(21)68, triggers at an allele-specific time to form small, heptameric lesions, or pinholes, in the cytoplasmic membrane, thus initiating lysis. S(21)68 has two transmembrane domains, TMD1 and TMD2. Only TMD2 is required for the formation of pinholes, whereas TMD1 acts as an inhibitor of TMD2 and must be externalized to the periplasm in the lytic pathway. Previously we provided evidence that S(21)68 first accumulates as inactive dimers with both transmembrane domains embedded in the bilayer. Here we analyse an extensive collection of S(21) mutants to identify residues and domains critical to the function and regulation of the pinholin. Evidence is presented indicating that, within the inactive dimer, TMD1 acts in trans as an inhibitor of the lethal function of TMD2. A wide range of phenotypes, from absolute lysis defectives to accelerated lysis triggering, are observed for mutations mapping to each topological domain. The pattern of phenotypes allows the generation of a model for the structure of the inactive dimer. The model identifies the faces of the two transmembrane domains involved in ...Continue Reading

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Oct 29, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ting PangRy Young
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Citations

Sep 3, 2013·Indian Journal of Microbiology·K V Srividhya, S Krishnaswamy
May 15, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ting PangRy Young
Jan 22, 2013·Journal of Bacteriology·Kam H ToRy Young
Mar 4, 2014·The Journal of Microbiology·Ryland Young
Feb 24, 2016·Frontiers in Microbiology·Clara RocesBeatriz Martínez
Oct 1, 2015·Microbiology·Amol Arunrao Pohane, Vikas Jain
Oct 10, 2012·FEMS Microbiology Reviews·Maria João CatalãoMadalena Pimentel
Jul 7, 2016·Journal of Bacteriology·Yi Chen, Ry Young
Apr 8, 2014·Journal of Bacteriology·Samir H MoussaRy Young
Aug 6, 2014·Journal of Bacteriology·Kam H To, Ry Young
Sep 8, 2010·Molecular Microbiology·Ting PangRy Young
Oct 30, 2013·Molecular Microbiology·Christos G SavvaRy Young
Jan 4, 2017·G3 : Genes - Genomes - Genetics·Jesse CahillRy Young
Nov 7, 2020·Proceedings of the National Academy of Sciences of the United States of America·Lena M E StegerAnne S Ulrich
Feb 1, 2014·Microbiology Spectrum·Madalena Pimentel
Dec 9, 2020·The Journal of Physical Chemistry. B·Tanbir AhammadGary A Lorigan

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