PMID: 2119552Jan 1, 1990Paper

Mutational analysis of the structure and function of GTP-binding proteins

Advances in Enzyme Regulation
S B MastersH R Bourne

Abstract

Structural, biochemical and molecular genetic studies of EF-Tu, p21ras and alpha s have begun to reveal the inner workings of the molecular machine used by these and other GTP-binding proteins. Further understanding of this molecular machine will ultimately come from crystal structures of the G protein alpha chains as well as from crystal structures of the GTP-bound forms of p21ras and EF-Tu. Mutational analysis will continue to add meaning to the static pictures provided by these crystal structures. Aside from their intrinsic biological interest, other reasons motivate our exploration of the GTP-dependent molecular machine used by GTP-binding proteins. Mutations or bacterial toxins cause disease by inhibiting the GTPase function of p21ras and alpha s. Other G protein alpha chains carry signals that regulate important cell functions, including proliferation. Malfunctions of these other G proteins are highly likely to cause disease. Applying our knowledge of p21ras and alpha s to these additional proteins may turn out to have significant practical consequences.

References

Sep 21, 1978·Biochimica Et Biophysica Acta·Y Kaziro
Jan 1, 1987·Molecular and Cellular Endocrinology·A M Spiegel
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M MatsuokaY Kaziro
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S R Holbrook, S H Kim
Jul 21, 1989·Science·L TongS H Kim
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D J ClantonT Y Shih
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J D RobishawA G Gilman
Sep 1, 1986·Molecular and Cellular Biology·C J DerG M Cooper
Jan 1, 1986·Annual Review of Cell Biology·L Stryer, H R Bourne
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H K FongM I Simon
Jan 1, 1988·Cold Spring Harbor Symposia on Quantitative Biology·H R BourneW Heideman
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J B GibbsE M Scolnick
Jan 1, 1987·Annual Review of Biochemistry·M Barbacid
Aug 8, 1986·Science·M WalterA D Levinson
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·I S SigalE M Scolnick
Feb 25, 2000·The International Journal of Biochemistry & Cell Biology·M G Coppolino, S Dedhar

❮ Previous
Next ❯

Citations

Jul 26, 2003·Protein Expression and Purification·Isabelle E LehouxChantal M Petit
Apr 24, 2010·Cardiovascular Toxicology·James H Zavecz, Harold D Battarbee
Feb 12, 1998·Neuron·L BelluscioR Axel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.