Mutational analysis of two highly conserved UGG sequences of 23 S rRNA from Escherichia coli.

The Journal of Biological Chemistry
C M SpahnK H Nierhaus

Abstract

The 23 S-type rRNA contains two phylogenetically conserved UGG sequences, which have the potential to bind the universal CCA-3'-ends of tRNAs at the ribosomal peptidyltransferase center by base pairing. The first two positions, UG, of these sequences at the helix-loop 80 (U2249G2250) and helix-loop 90 (Psi2580G2581) and some related nucleotides were tested by site-directed mutagenesis for their involvement in ribosomal function, i.e. peptidyltransferase. The plasmid-derived mutated 23 S rRNA comprised about 50% of the total 23 S rRNA. None of the single mutations caused an assembly defect, and all 50 S subunits carrying an altered 23 S rRNA could freely exchange with the pools of 70S ribosomes and polysomes. The mutations at the helix-loop 80 region hardly affected bacterial growth. However, mutations at the helix 90 caused severe growth effects and severely impaired the in vitro protein synthesis, showing that this 23 S rRNA region is of high importance for ribosomal function.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jun 25, 1992·Nucleic Acids Research·U Saarma, J Remme
Jan 1, 1991·Annual Review of Biochemistry·H F Noller
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D Moazed, H F Noller
Jan 1, 1988·Methods in Enzymology·C D SigmundE A Morgan
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Jul 28, 1968·Journal of Molecular Biology·B E MadenR E Monro
Oct 3, 1983·European Journal of Biochemistry·S B BourdA A Krayevsky
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A BartaE Kuechler
Feb 1, 1982·European Journal of Biochemistry·E G WagnerC G Kurland
Jun 1, 1995·European Journal of Biochemistry·R Brimacombe
Sep 1, 1993·Journal of Bacteriology·H F Noller
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·M Liu, J Horowitz
Jul 1, 1993·Nucleic Acids Research·R R GutellM N Schnare
Dec 20, 1996·The Journal of Biological Chemistry·C M SpahnK H Nierhaus

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Citations

Apr 9, 1999·Biological Chemistry·S Dorner, A Barta
Jan 5, 2000·Molecular Cell·D F Kim, R Green
Dec 20, 1996·The Journal of Biological Chemistry·C M SpahnK H Nierhaus
Jun 23, 1998·The Journal of Biological Chemistry·M S IordanovB E Magun
Apr 23, 2002·The Journal of Biological Chemistry·Kai VirumaeJaanus Remme
Sep 18, 2009·PloS One·Mark WelchClaes Gustafsson
Aug 15, 1998·Biological Chemistry·C M Spahn, K H Nierhaus
Jul 30, 1999·Biochemical and Biophysical Research Communications·T OberholzerP L Luisi
Dec 15, 2000·Journal of Molecular Biology·R WillumeitK H Nierhaus
Mar 25, 2019·Journal of the American Chemical Society·Larisa M Dedkova, Sidney M Hecht
Aug 23, 2021·Journal of Molecular Biology·Sidney M Hecht
Jul 12, 2014·BMC Research Notes·Jeanneth Mosquera-RendónAlfonso Benítez-Páez
Aug 21, 2015·RNA·Yulia V GerasimovaDmitry M Kolpashchikov

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