Mutational analysis of two highly conserved motifs in the silencing suppressor encoded by tomato spotted wilt virus (genus Tospovirus, family Bunyaviridae)

Archives of Virology
Ying ZhaiHanu Pappu

Abstract

Tospoviruses cause serious economic losses to a wide range of field and horticultural crops on a global scale. The NSs gene encoded by tospoviruses acts as a suppressor of host plant defense. We identified amino acid motifs that are conserved in all of the NSs proteins of tospoviruses for which the sequence is known. Using tomato spotted wilt virus (TSWV) as a model, the role of these motifs in suppressor activity of NSs was investigated. Using site-directed point mutations in two conserved motifs, glycine, lysine and valine/threonine (GKV/T) at positions 181-183 and tyrosine and leucine (YL) at positions 412-413, and an assay to measure the reversal of gene silencing in Nicotiana benthamiana line 16c, we show that substitutions (K182 to A, and L413 to A) in these motifs abolished suppressor activity of the NSs protein, indicating that these two motifs are essential for the RNAi suppressor function of tospoviruses.

References

Jun 23, 1998·The Plant Cell·M T RuizD C Baulcombe
Jul 27, 2000·Molecular and Cellular Biology·J J DerryA L Tyner
Jun 26, 2001·Science·V Vance, H Vaucheret
Mar 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sang-Hoon SinJames W Moyer
Aug 5, 2005·Annual Review of Phytopathology·Anna E WhitfieldThomas L German
May 26, 2006·The EMBO Journal·Lóránt LakatosJózsef Burgyán
Aug 6, 2008·Annual Review of Phytopathology·Juan A Díaz-Pendón, Shou-Wei Ding
Mar 23, 2010·PloS One·Bhushan LokeshHandanahal S Savithri
May 1, 2000·Molecular Plant Pathology·S Adkins
Mar 29, 2011·Trends in Plant Science·József Burgyán, Zoltán Havelda
Apr 12, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Andreas PfannkucheJohannes Georg Bode
Oct 25, 2011·Molecular Plant Pathology·Karen-Beth G ScholthofGary D Foster
Jun 12, 2012·Advances in Virus Research·Massimo TurinaMarina Ciuffo
Jun 21, 2012·Molecular Plant-microbe Interactions : MPMI·Ana P LunaEduardo R Bejarano

❮ Previous
Next ❯

Citations

Sep 1, 2016·Annual Review of Virology·J E Oliver, A E Whitfield
Apr 6, 2019·Critical Reviews in Biotechnology·Ashish PrasadManoj Prasad
Jul 23, 2020·Frontiers in Plant Science·Ying ZhaiHanu R Pappu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.