PMID: 3320958Dec 10, 1987Paper

Mutational analysis of upstream sequences required for transcriptional activation of the Klebsiella pneumoniae nifH promoter

Nucleic Acids Research
M BuckJ Woodcock

Abstract

Upstream sequences of the Klebsiella pneumoniae nifH promoter were mutagenised and activation of the mutated promoters by the nif-specific transcriptional activator protein NifA examined in vivo. Of the sixteen mutations analysed, only those within the nifH upstream activator sequence (UAS), characterised by a TGT-N10-ACA motif, influenced nifH promoter activity. Mutations altering the two-fold rotational symmetry of the UAS or the spacing between the TGT and ACA motifs reduced promoter activity, consistent with the UAS functioning as a NifA binding site. The bases flanking the TGT-ACA motif of the UAS also appear to influence activation by NifA. Substituting the nifH UAS with a binding site for the transcriptional activator NtrC resulted in improved NtrC-dependent activation of the nifH promoter demonstrating that the activator specificity of the nifH promoter is dependent upon the presence of the appropriate upstream sequences to which the activator binds.

References

Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T P Hunt, B Magasanik
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A J Ninfa, B Magasanik
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J HirschmanS Kustu
Jan 1, 1986·Annual Review of Genetics·G N GussinF M Ausubel
Jan 1, 1982·Annual Review of Genetics·B Magasanik

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Citations

Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E Morett, M Buck
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·B BritoT Ruiz-Argüeso
Oct 1, 1990·Molecular Microbiology·H Hennecke
May 24, 2006·Journal of Bioscience and Bioengineering·Hisayoshi TsuihijiMikio Kataoka
Sep 1, 1994·Microbiological Reviews·H M Fischer

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