Mutational analysis of vaccinia virus E3 protein: the biological functions do not correlate with its biochemical capacity to bind double-stranded RNA

Journal of Virology
Kevin J DueckJingxin Cao

Abstract

Vaccinia E3 protein has the biochemical capacity of binding to double-stranded RNA (dsRNA). The best characterized biological functions of the E3 protein include its host range function, suppression of cytokine expression, and inhibition of interferon (IFN)-induced antiviral activity. Currently, the role of the dsRNA binding capacity in the biological functions of the E3 protein is not clear. To further understand the mechanism of the E3 protein biological functions, we performed alanine scanning of the entire dsRNA binding domain of the E3 protein to examine the link between its biochemical capacity of dsRNA binding and biological functions. Of the 115 mutants examined, 20 were defective in dsRNA binding. Although the majority of the mutants defective in dsRNA binding also showed defective replication in HeLa cells, nine mutants (I105A, Y125A, E138A, F148A, F159A, K171A, L182A, L183A, and I187/188A) retained the host range function to various degrees. Further examination of a set of representative E3L mutants showed that residues essential for dsRNA binding are not essential for the biological functions of E3 protein, such as inhibition of protein kinase R (PKR) activation, suppression of cytokine expression, and apoptosis. Th...Continue Reading

References

Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H W ChangB L Jacobs
Oct 1, 1995·Journal of Virology·H W ChangB L Jacobs
Jan 25, 2003·Annual Review of Immunology·Bruce T SeetGrant McFadden
Jun 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yang-Gyun KimAlexander Rich
Dec 15, 2004·Journal of Virology·Bianca T Hovey NerenbergKlaus Früh
Apr 28, 2005·The FEBS Journal·Kung-Yao Chang, Andres Ramos
Jul 1, 2008·Advances in Virus Research·Steven J WerdenGrant McFadden
Aug 28, 2009·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Beatriz Perdiguero, Mariano Esteban
Jun 4, 2010·The Journal of General Virology·Robert Valentine, Geoffrey L Smith

❮ Previous
Next ❯

Citations

Aug 9, 2017·Viruses·Daniel Brian NicholsJessica Cottrell
Sep 12, 2015·Journal of Virology·Yeu-Yang TsengWei-Li Hsu
Nov 2, 2018·Wiley Interdisciplinary Reviews. RNA·Nathan MeadeDerek Walsh
Feb 23, 2021·Frontiers in Immunology·De Chen ChiangSiew Kit Ng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.