Abstract
The ability of Pseudomonas fluorescens F113 to produce the antibiotic 2,4-diacetylphloroglucinol (DAPG) is a key factor in the biocontrol of the phytopathogenic fungus Pythium ultimum by this strain. In this study, a DAPG-producing strain (rifampin-resistant mutant F113Rif) was compared with a nearly isogenic DAPG-negative biosynthesis mutant (Tn5::lacZY derivative F113G22) in terms of the ability to colonize and persist in the rhizosphere of sugarbeets in soil microcosms during 10 plant growth-harvest cycles totaling 270 days. Both strains persisted similarly in the rhizosphere for 27 days, regardless of whether they had been inoculated singly onto seeds or coinoculated in a 1:1 ratio. In order to simulate harvest and resowing, the roots were removed from the soil and the pots were resown with uninoculated sugarbeet seeds for nine successive 27-day growth-harvest cycles. Strains F113Rif and F113G22 performed similarly with respect to colonizing the rhizosphere of sugarbeet, even after nine cycles without reinoculation. The introduced strains had a transient effect on the size of the total culturable aerobic bacterial population. The results indicate that under these experimental conditions, the inability to produce DAPG did no...Continue Reading
References
Dec 1, 1992·Applied and Environmental Microbiology·A M FentonF O'Gara
Aug 1, 1992·Applied and Environmental Microbiology·M MazzolaL S Pierson
Dec 21, 1989·Gene·Y P WangF O'Gara
Aug 1, 1988·Journal of Bacteriology·L S Thomashow, D M Weller
Jun 1, 1987·Journal of Bacteriology·L A De WegerB Lugtenberg
Oct 1, 1984·Applied and Environmental Microbiology·D M Weller
Feb 1, 1985·Applied and Environmental Microbiology·J E LoperM N Schroth
Apr 1, 1990·Applied and Environmental Microbiology·L S ThomashowL S Pierson
Aug 1, 1990·Applied and Environmental Microbiology·Ron van PeerBob Schippers
Jan 1, 1992·Applied and Environmental Microbiology·P ShanahanF O'gara
Citations
Jul 27, 2001·Applied and Environmental Microbiology·Y Moënne-LoccozF O'Gara
Oct 24, 2008·Phytopathology·Blanca B LandaDavid M Weller
Feb 24, 2012·PloS One·Guanpeng GaoWei Wang
Mar 10, 2004·Applied and Environmental Microbiology·Brion DuffyGeneviève Défago
Aug 10, 2000·Microbiology·C DunneF O'Gara
May 6, 2003·Annual Review of Phytopathology·Dieter Haas, Christoph Keel
May 26, 2001·Applied and Environmental Microbiology·J M Raaijmakers, D M Weller
Oct 24, 2008·Phytopathology·Jorge T de SouzaJos M Raaijmakers
Apr 23, 2017·Applied and Environmental Microbiology·Mélanie M PaulinMartin Filion
Apr 25, 2007·Applied Microbiology and Biotechnology·S MizumotoM Shoda
Jun 3, 1998·Applied and Environmental Microbiology·J M BareaC Azcon-Aguilar
Apr 12, 2001·Journal of Applied Microbiology·D C NasebyJ M Lynch
Aug 1, 2004·FEMS Microbiology Ecology·Stacey Blouin BankheadBrian B McSpadden Gardener
Dec 19, 2012·Bioengineered·Colum Dunne
May 29, 2018·The Journal of General and Applied Microbiology·Wangtai LuoYihe Ge
Dec 1, 1997·Microbiology·Colum DunneFergal O'Gara
Mar 1, 2003·The New Phytologist·Thomas F C Chin-A-WoengBen J J Lugtenberg
Nov 1, 1996·Applied and Environmental Microbiology·O Asaka, M Shoda
Jan 1, 1997·Applied and Environmental Microbiology·J TroxlerD Haas
Apr 1, 1997·Applied and Environmental Microbiology·D CroninF O'gara
Nov 15, 1997·Applied and Environmental Microbiology·S FediF O'Gara
Mar 15, 2006·Applied and Environmental Microbiology·J TroxlerG Defago