Mutational specificity and genetic control of replicative bypass of an abasic site in yeast.

Proceedings of the National Academy of Sciences of the United States of America
Vincent PagèsSatya Prakash

Abstract

Abasic (AP) sites represent one of the most frequently formed lesions in DNA, and they present a strong block to continued synthesis by the replicative DNA polymerases (Pols). Here we determine the mutational specificity and the genetic control of translesion synthesis (TLS) opposite an AP site in yeast by using a double-stranded plasmid system that we have devised in which bidirectional replication proceeds from a replication origin. We find that the rate, the genetic control, and the types and frequencies of nucleotides inserted opposite the AP site are very similar for both the leading and the lagging DNA strands, and that an A is predominantly inserted opposite the AP site, whereas C insertion by Rev1 constitutes a much less frequent event. In striking contrast, in studies that have been reported previously for AP bypass with gapped-duplex and single-stranded plasmids, it has been shown that a C is the predominant nucleotide inserted opposite the AP site. We discuss the implications of our observations for the mechanisms of TLS on the leading versus the lagging DNA strand and suggest that lesion bypass during replication involves the coordination of activities of the replicative Pol with that of the lesion-bypass Pol.

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Citations

Mar 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kevin Lehner, Sue Jinks-Robertson
Jun 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Narottam AcharyaSatya Prakash
Jun 20, 2012·Proceedings of the National Academy of Sciences of the United States of America·Robert E JohnsonSatya Prakash
Sep 25, 2009·The Journal of Biological Chemistry·Nasim Sabouri, Erik Johansson
Sep 21, 2013·Nucleic Acids Research·Matthew R NorthamPolina V Shcherbakova
Sep 14, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Barbara L ParsonsMartha M Moore
Jan 13, 2010·Molecular and Cellular Biology·Knut H LauritzenArne Klungland
Apr 18, 2013·PLoS Genetics·Jennifer A CalvoLeona D Samson
Jan 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Celine WalmacqMikhail Kashlev
Apr 3, 2016·Mutation Research. Reviews in Mutation Research·Alice Machado-SilvaCarlos Renato Machado
Dec 21, 2010·Journal of Molecular Biology·Deepak T NairAneel K Aggarwal
Mar 25, 2016·DNA Repair·A John Callegari
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Mar 24, 2017·PloS One·Daniel E EylerPatrick J O'Brien
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Mar 1, 2018·Proceedings of the National Academy of Sciences of the United States of America·Wei WangDong Wang
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Apr 3, 2013·Genetics·Serge Boiteux, Sue Jinks-Robertson
Aug 17, 2019·Nucleic Acids Research·Katarzyna H MasłowskaVincent Pagès
Jan 16, 2010·Biochemical Society Transactions·Alfonso Gallego-SánchezAvelino Bueno
Apr 12, 2011·Chembiochem : a European Journal of Chemical Biology·Samra ObeidAndreas Marx
Jan 16, 2010·Biochemical Society Transactions·Abel C S Chun, Dong-Yan Jin

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