Mutational specificity of animal cell DNA polymerases

Environmental Mutagenesis
J D Roberts, T A Kunkel

Abstract

Since DNA polymerases are involved in DNA replication, recombination, and repair, the frequency with which these enzymes commit errors during synthesis is likely to be an important factor in controlling mutation rates in cells. The fidelity of DNA polymerases was originally studied by following misincorporation using synthetic nucleic acid templates containing only one or two bases. Later, by assaying for reversion of an amber codon after copying phi X174 single-stranded DNA molecules, the base substitution accuracy of in vitro DNA synthesis on natural DNA was determined. Most recently, a forward mutation assay has been developed that uses gap-filling synthesis on an M13mp2 DNA template, thus permitting the detection of a variety of different errors during DNA synthesis on natural DNA templates. Detailed mutational spectra for animal cell polymerases-alpha, beta, and gamma have been determined and demonstrate that a variety of errors can be generated by these purified enzymes. The frequencies of base mispairs, base additions, and deletion errors by DNA polymerases vary widely and depend on both the DNA sequence and the enzyme used. An understanding of the mechanisms by which DNA polymerases avoid or generate various mutations d...Continue Reading

References

Dec 1, 1979·Archives of Biochemistry and Biophysics·A Weissbach
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M MeuthM Trudel
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L A Weymouth, L A Loeb
Jan 1, 1978·Annual Review of Biochemistry·R Sheinin, J Humbert
Feb 15, 1977·European Journal of Biochemistry·B OttoR Knippers
Jan 11, 1985·Nucleic Acids Research·J Abbotts, L A Loeb
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J D HallC W Knopf
Jan 29, 1985·Biochimica Et Biophysica Acta·J Abbotts, L A Loeb
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J PetruskaM F Goodman
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M WablC Steinberg
Jan 1, 1974·Annual Review of Biochemistry·A S Mildvan
Aug 1, 1973·The Journal of Cell Biology·R Hand, I Tamm
Mar 22, 1969·Nature·J W Drake
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J J Hopfield
Jan 1, 1974·Molecular & General Genetics : MGG·R G FowlerE C Cox
Jul 1, 1984·Molecular and Cellular Biology·K R TindallA W Hsie
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·W S El-DeiryA G So
Jan 1, 1982·Annual Review of Biochemistry·L A Loeb, T A Kunkel
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R M SchaaperL A Loeb
Jul 5, 1983·Journal of Molecular Biology·J NalbantogluM Meuth
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E KarawyaS H Wilson
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P K LiuL A Loeb
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J Shlomai, A Kornberg
Aug 14, 1981·Science·T A Kunkel, L A Loeb
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M P CalosM R Botchan
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·E BarilP Zamecnik
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P LamotheE Baril
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S M Watanabe, M F Goodman
May 30, 1953·Nature·J D WATSON, F H CRICK
Mar 1, 1960·Proceedings of the National Academy of Sciences of the United States of America·J R Fresco, B M Alberts

❮ Previous
Next ❯

Citations

Jun 9, 1998·Journal of Cellular Physiology·S KitanoG S Roth
May 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K M DowneyA G So
Apr 1, 1990·Journal of Cellular Physiology·J M Collins, A K Chu
Dec 1, 1991·Molecular Reproduction and Development·J A LawittsJ D Biggers
Sep 1, 1996·Plant Molecular Biology·J P BenedettoM Castroviejo
Feb 1, 1992·Molecular Biology Reports·T S BalmukhanovN A Ajtkhozhina
Jan 1, 1990·Radiation and Environmental Biophysics·S G SwartsK T Wheeler
Jan 1, 1991·Somatic Cell and Molecular Genetics·P K Liu, T H Norwood
Mar 4, 1992·Molecular and Cellular Biochemistry·P Zahradka
Jan 1, 1996·Parasitology Research·A MakiokaT Takeuchi
Jul 31, 1989·Clinica Chimica Acta; International Journal of Clinical Chemistry·U BertazzoniE Cattaneo
Jul 1, 1993·The International Journal of Biochemistry·V K SrivastavaD L Busbee
Mar 1, 1993·The International Journal of Biochemistry·V K SrivastavaD L Busbee
Jul 1, 1994·International Journal for Parasitology·A Makioka, J Ellis
Dec 1, 1991·Mutation Research·R Maldonado-RodriguezK L Beattie
Jan 1, 1990·Pharmacology & Therapeutics·R V Talanian, G E Wright
Jan 1, 1990·Pharmacology & Therapeutics·G E Wright, N C Brown
Apr 1, 1991·Molecular and Biochemical Parasitology·E de VriesJ P Overdulve
Jan 17, 1991·Biochimica Et Biophysica Acta·R A Bambara, C B Jessee
Jan 6, 1992·Biochimica Et Biophysica Acta·O Popanda, H W Thielmann
Jan 1, 1989·Experimental Gerontology·D W Smith, H R Warner
Jan 1, 1991·Experimental Gerontology·V K SrivastavaD L Busbee
Jan 1, 1991·Experimental Gerontology·V K SrivastavaD Busbee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.