Mutational spectra in transgenic animal research: data analysis and study design based upon the mutant or mutation frequency

Environmental and Molecular Mutagenesis
G J Carr, N Gorelick

Abstract

Understanding chemically induced changes in mutational spectra can aid in deciphering mechanisms of mutagenesis. In this paper, we propose the use of statistical methods that are based upon the mutation frequency, rather than simple mutant counts which have no relationship to the mutation frequency. These methods have a number of advantages over the current standard analysis: an improved means of identifying those classes/sites of mutation which have treatment-related induction, greater sensitivity to localized differences in spectra (e.g., limited to a single base pair), one-sided tests for induction of mutations, tests of dose-response, and a framework for sample-size estimation in terms of the number of mutants to sequence. As examples, the methods are applied to data from transgenic mutation assays.

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Citations

Oct 11, 2003·Mutation Research·Véronique ThybaudNobuhiro Yajima
Dec 18, 2013·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Yukari TotsukaHitoshi Nakagama
Dec 11, 1999·Mutation Research·N N Khromov-BorisovF J de Serres
Aug 6, 2005·Mutation Research·Iain B LambertGeorge R Douglas
Jan 1, 1996·Environmental and Molecular Mutagenesis·N F Cariello, N J Gorelick
Dec 19, 2002·Environmental and Molecular Mutagenesis·Henriqueta LouroMaria G Boavida
Jan 9, 1999·Environmental and Molecular Mutagenesis·J N ThompsonH Huai
Feb 17, 2001·Human Mutation·I RogozinG Glazko
Mar 18, 2014·Nanomaterials·Yukari TotsukaKeiji Wakabayashi
Nov 7, 2000·Genetics·D B Dunson, K R Tindall

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