Feb 21, 2018

Mutational studies of the Aux/IAA proteins in Physcomitrella reveal novel insights into their function

The New Phytologist
Sibo Tao, Mark Estelle

Abstract

The plant hormone auxin regulates many aspects of plant growth and development. Auxin signaling involves hormone perception by the TRANSPORT INHIBITOR RESPONSE/AUXIN F-BOX (TIR1/AFB)-Aux/IAA co-receptor system, and the subsequent degradation of the Aux/IAA transcriptional repressors by the ubiquitin proteasome pathway. This leads to the activation of downstream gene expression and diverse physiological responses. Here, we investigate how the structural elements in the Aux/IAAs determine their function in Auxin perception and transcriptional repression. We took advantage of the facile genetics of the moss Physcomitrella patens to determine the activity of wild-type and mutant PpIAA1a proteins in a Δaux/iaa null background. In this way, Aux/IAA function was characterized at the molecular and physiological levels without the interference of genetic redundancy. We identified and characterized degron variants in Aux/IAAs that affect their stability and Auxin response. We also demonstrated that neither the Aux/IAA EAR motif nor Aux/IAA oligomerization is essential for the repressive function of Aux/IAA. Our study demonstrates how key elements within the Aux/IAA proteins fine tune stability and repressor activity, as well as the long-...Continue Reading

  • References27
  • Citations1

References

  • References27
  • Citations1

Citations

Mentioned in this Paper

Study
Biochemical Pathway
Proteasome Pathway
Nuclear Receptor Subfamily 1, Group D, Member 1
Tune Deafness
Genes, Plant
Transcription Repressor/Corepressor
Ubiquitin
Transcription, Genetic
Tissue Specificity

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