Abstract
The Brazilian public health system (SUS) has provided antiviral drugs for chronic hepatitis B treatment for over 10 years, but a system for monitoring for drug-related resistance mutations is not available. Determine the presence of HBV mutations associated with resistance to nucleos(t)ide analogs among 81 patients with chronic HBV infection in Salvador-BA-Brazil. HBV-DNA was PCR amplified with primers deduced from the rt domain at the HBV P gene, the sequence extended 1032 bp (from amino acid 1 to 344-rt domain). Those sequences were submitted to the HBV drug resistance database to retrieve each mutation according to the genotype. HBV genotype A1 (85.2%) was the most prevalent, followed by genotype A2 (4.9%), F (6.2%), and C1, D2, and D4 (1.2% each). Six patients (7%) exhibited resistance mutations to LAM, ETV, and TDF: two with patterns L180M + M204V and four with other different patterns: L80I + L180M + M204I; L80V + L180M + M204V; M204I; A194T. All of these mutations were present in patients with genotype A (four A1 and two A2). In addition, four mutations in gene S (three cases with the sI195M mutation and one with the W196L mutation), were detected, corresponding to a rate of 6% of vaccine escape mutations. Althougth the ...Continue Reading
References
May 1, 1992·Journal of Virology·M MartellJ Gómez
Jan 1, 1995·Vaccine·M Kane
Jan 21, 2000·The Journal of General Virology·L StuyverR Rossau
Sep 1, 2000·Revista Do Instituto De Medicina Tropical De São Paulo·L C Da SilvaJ R Pinho
Nov 2, 2001·International Journal of Epidemiology·R Westerling
Aug 14, 2002·World Journal of Gastroenterology : WJG·Song Chen, Yu-Ming Wang
Jan 31, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Bernhard ZöllnerHeinz-Hubert Feucht
Jun 29, 2004·Archives of Virology·N M AraujoS A Gomes
Jul 5, 2007·Journal of Gastroenterology and Hepatology·Alexander J V ThompsonPaul V Desmond
Jan 24, 2008·BMC Microbiology·Marcelle BottecchiaSelma A Gomes
Mar 6, 2008·Nature Reviews. Genetics·Siobain DuffyEdward C Holmes
Jun 22, 2010·Revista Da Sociedade Brasileira De Medicina Tropical·Rodrigo HaddadJonny Yokosawa
Sep 30, 2010·Antiviral Research·Soo-Yon RheeRobert W Shafer
Apr 28, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Dennis Armando BertoliniJoão Renato Rebello Pinho
Mar 8, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Karine LacombeFabien Zoulim
Jan 28, 2015·Antiviral Therapy·Michele S Gomes-GouvêaJoão R Rebello Pinho
Citations
Jan 19, 2020·International Journal of Molecular Sciences·Md Golzar HossainKeiji Ueda
Dec 13, 2018·Virusdisease·Modhusudon ShahaMd Salimullah
Aug 8, 2019·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Chao LiuLunan Wang
Mar 25, 2021·Infection and Drug Resistance·Jing LiXinxin Zhang
May 24, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Jonas Michel WolfVagner Ricardo Lunge
Aug 11, 2021·Viruses·Ronylson José S SilvaAldemir B Oliveira-Filho