PMID: 9420055Jan 7, 1998Paper

Mutations at codon 184 in simian immunodeficiency virus reverse transcriptase confer resistance to the (-) enantiomer of 2',3'-dideoxy-3'-thiacytidine

Antimicrobial Agents and Chemotherapy
E CherryM A Wainberg

Abstract

Variants of simian immunodeficiency virus (SIV) that display greater than 2,000-fold resistance to the (-) enantiomer of 2',3'-dideoxy-3'-thiacytidine (3TC) were generated through in vitro passage and drug selection. The polymerase regions of several of these resistant viruses were sequenced and were found to share either of two codon alterations at site 184 in reverse transcriptase (ATG to ATA [methionine to isoleucine] and ATG to GTA [methionine to valine]). The biological relevance of these substitutions for 3TC was confirmed by site-directed mutagenesis with the SIVmac239 infectious recombinant clone of SIV.

References

Nov 1, 1990·AIDS Research and Human Retroviruses·D A Regier, R C Desrosiers
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B A LarderD J Purifoy
Jul 1, 1995·Antimicrobial Agents and Chemotherapy·P L Boyer, S H Hughes
Apr 1, 1993·Antimicrobial Agents and Chemotherapy·R F SchinaziJ W Mellors
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M TisdaleB A Larder

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Citations

Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·S G SarafianosE Arnold
Oct 25, 2003·Antimicrobial Agents and Chemotherapy·Karidia DialloM A Wainberg

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