Mutations at positions 186 and 194 in the HA gene of the 2009 H1N1 pandemic influenza virus improve replication in cell culture and eggs.

Virology Journal
Pirada SuphaphiphatPeter W Mason

Abstract

Obtaining suitable seed viruses for influenza vaccines poses a challenge for public health authorities and manufacturers. We used reverse genetics to generate vaccine seed-compatible viruses from the 2009 pandemic swine-origin influenza virus. Comparison of viruses recovered with variations in residues 186 and 194 (based on the H3 numbering system) of the viral hemagglutinin showed that these viruses differed with respect to their ability to grow in eggs and cultured cells. Thus, we have demonstrated that molecular cloning of members of a quasispecies can help in selection of seed viruses for vaccine manufacture.

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Citations

Aug 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Daphna MerozNir Ben-Tal
May 31, 2013·Journal of Virology·Steven F BakerLuis Martínez-Sobrido
May 17, 2013·Science Translational Medicine·Philip R DormitzerJ Craig Venter
Nov 3, 2012·Enfermedades infecciosas y microbiología clínica·Andrés AntónTomás Pumarola
Aug 17, 2014·The Journal of General Virology·Alessio LorussoAmy L Vincent
Jan 21, 2015·Veterinary Microbiology·Jamie N HenningsonAmy L Vincent
Nov 4, 2017·Scientific Reports·Christopher S AndersonMarta L DeDiego

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Datasets Mentioned

BETA
GQ117044
FJ969517

Methods Mentioned

BETA
PCR
transfections
transfection

Software Mentioned

Chimera

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